| Project/Area Number |
04660015
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
作物
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| Research Institution | Kobe University |
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Principal Investigator |
YASUDA Takeshi Kobe University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (20026553)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Tadashi Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (50031216)
AZUMA Tetsushi Kobe University, The Graduate School of Science & Technology, Assistant Professo, 大学院自然科学研究科, 助手 (30231913)
UCHIDA Naotugu Kobe University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (70151884)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Somatic embryo / Coffee / Cryopreservation / Cytokinin / Protoplast / Tropical Tree / Coffea / 凍結保存 |
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| Research Abstract |
The embryogenic callus and somatic embryo of Coffea arabica and C.canephora can be induced by cytokinin alone from the section of young leaf of mature trees. The cytokinin induced callus has a high embryogenic potetial and is very useful for clonal propagation. As cytokinin, 2-isopentenyl-adenine(2iP)is the best, and benzyladenin(BA), zeatin and kinetin also are effective for somatic embryogenesis from coffee leaf slices. Additional auxins act inhibitory effect on somatic embryogenesis. Protoplast were prepared from embryogenic calluses from C.arabica and canephora and cultured on Gelrite medium with cytokinin as a plant regulator. Somatic embryos were regenerated from protoplasts. These simplified method for preparation and culture of protoplasts is very useful for cell fusion and genetic engeering. Somatic embryos of Coffea were successfully cryopreserved after being exposed to a series of preparative steps ; precultured on media with increasing concentration of sucrose, encapsulated in alginate beads, dehydrated up to 30% and pluged into liquid nitrogen.
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