| Project/Area Number |
04660065
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
土壌・肥料
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| Research Institution | Yamagata University |
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Principal Investigator |
WAGATSUMA Tadao Yamagata University Department of Agriculture Professor, 農学部, 教授 (70007079)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Toshinori Yamagata University Department of Agriculture Associate professor, 農学部, 助教授 (80202670)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Al tolerance / Plasma membrane / Protoplast / Synthesis of beta-glucan / Release of organic acid / La^<3+> / Yb^<3+> / Silica microbeads / グルカン / イツテルビウム |
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| Research Abstract |
(1) Elucidation of the primary specific site for Al^<3+> : Experiments were carried out by utilizing the difference of the order of ionic potential ; Al^<3+>>Yb^<3+>>La^<3+>. The amount of beta-glucan synthesizd on the surface of protoplasts from root tip was in the order of Al>Yb>La ; a universal deposition of beta-glucan on that from Al-tolerant, but a heterogeneous dotted deposition from al-sensitive plant species. The beta-glucan synthesized was composed in part of 1, 3-beta-glucan. FDA viability of root treated with metals was in the order of Al<Yb<La, and the accumulation of metals in the outer portions of roots was considerable in the order of Al>Yb>La. La induced most heavily the irreversible plasmolysis, the inhibition of root elongation, and the loss of K from roots. The aggregation of protoplasts in non-synthesizing conditions of beta-glucan was higher in the order of Al>Yb>La. These results indicate the plasma membrane of the outer and tip portions of roots as the primary specific site for Al^<3+>. (2) Isolation of Al-tolerant root cells based on the surface negativity of plasma membrane from root tip : Al tolerance was not correlated with CEC of root tip, but megatively correlated with the negativity of plasma membrane. Non-toxic Al(OH)_3 was mixed with silica microbeads (Al-SMB) ; the pH of the highest positive charge of Al-SMB was 5.3. Purified protoplasts from root tip were mixed with Al-SMB at this pH, and finally viable Al-tolerant protoplasts were isolated predominantly in the bottom fraction by centrifuging with a discontinuous gradient. (3) Preparation of solid medium without Al and the analysis of organic acids released from roots : By adding the nutrient solution to SMB, agar-like solid medium without Al was prepered ; physical properties of it resembled the actual soil conditions. Organic acids released from roots were collected by filtering the Al-SMB.(4) Relationship between Al olerance and organic acids rel
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