| Project/Area Number |
04660069
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
土壌・肥料
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
MATOH Toru Kyoto University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (50157393)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Boron / Boric acid / Pectic substances / Rhammnogalacturonan / Radish / 細胞壁 / ニジトロナフタレンジオール / タバコ / 培養細胞 |
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| Research Abstract |
Crude cell walls were prepared from radish roots with grading, ethanol- and methanol/chloroform-washing and freeze-drying. The boron-containing polysaccharide was solubilized and purified from the crude radish cell walls by pectinase digestion and subsequent column chromatography on a DEAE-Toyopearl, Superdex-75 and DEAE-Sephacel. The purified polysaccharide had a molecular weight of 7,000 and comprised of uronic acid 52%, abarinose 17.4%, galactose 4.9% and rhammnose 9.8%, suggesting the boron-containing polysaccharide was a Rhammnogalacturonan. One molecule of the polysaccharide contained one molecule of boron as boric acid, as revealed with 11B NMR.Recovery of boron in this polysaccharide amounted to 80% of the boron of the cell walls, indicating that boron occurs in cell walls as a bound form almost exclusively with this polysaccharide. I speculate that boron may fulfill its function through binding with this polysaccharide.
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