| Project/Area Number |
04660114
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用微生物学・発酵学
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| Research Institution | Mie University |
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Principal Investigator |
SHIMADA Kyo Mie University Faculty of Bionresources Professor, 生物資源学部, 教授 (20024549)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | acid resistant xylanase / thermo-alkaline resitant xylanase / bleaching of kraft pulp / acid resistant penicillium / thermophilic anaerobes / 耐熱性キシラナーゼ / 耐酸性キシロシダーゼ / C、stercorarium / Penicillium sp.40 / キシラナーゼ遺伝子 / 短鎖化キシラナーゼ / 脱リグニン作用 |
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| Research Abstract |
The production of an acid resistant cylanase and a thermo-alkaline resistant xylamase were studied. Using these euzymes, the bleachability of xylanase-treated kraft pulp was extensively invetigated.
1. The acid resistant xylanase was produce from an acid-tolerant penicillium on the acidic culture cindition (ph2.0). Addition of inducers was promote the production of the xylanase.
2. The crude enzyme from the culture filtrate of the acid-tolerant penicillium was not include the cellulase. So the crude xylanase preparation was applied as one stage of bleaching. The results of our studies showed that xylanase treatment was very effective in improving bleachability of kraft pulp.
3. The xylanase gene of the thermophilic and anaerobic bacterium clostridium stercorarium F-9 cloned into Escherichia coli JM109. The translates product (xylanase) showed weak activity against cellulose, strong thermostability and alkaline stable nature. The treatment of this xylanase was no effect on improvement of pulp bleachability.
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