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Evolutionary relationship of biphenyl-catabolic genes in soil bacteria

Research Project

Project/Area Number 04660124
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・発酵学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

FURUKAWA Kensuke  Kyushu University, Department of Argricultural Chemistry, Associate Professor, 農学部, 助教授 (90221556)

Co-Investigator(Kenkyū-buntansha) YOSHINO Sadazou  Kyushu University, Department of Argricultural Chemistry, Assitant Professor, 農学部, 助手 (80117291)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsBiphenyl-utilizing bacteria / Toluene-utilizing bacteria / Catabolic genes / Nucleotide sequence / Molecular evolution / Genetic homology / Dioxygenase / Substrate specificity / ビフェニル / 土壌細菌 / コスミドライブラリー
Research Abstract

Biphenyl(BP) utilizing bacteria are widely distributed in the environment. Thirty two different BP-strains isolated far clasified into four groups according to the homologies of BP-catabolic bph genes and immunological properties of BP-ring-cleavage dioxygenases. The bph operon of Pseudomonas pseudoalcaligenes KF707 is organized as bphA1A2A3A4BCXD.Biphenyl dioxygenase is a multi-component enzyme which composed of large subunit(encoded by bphA1) and small subunit(encoded by bphA2)of terminal deioxygenase, ferredoxin(encoded by bphA3)and ferredoxin reductase(encoded by bphA4). bphB encodes BP-dihydrodiol dehydrogenase, bphC encodes BP-ring meta-cleavage dioxygenase, and bphD encodes hydrolase. The bph operon is very similar to toluene catabolic tod operon of Pseudomonas putida F1 in the gene organization and nucleotide sequences. The gene components responsible for substrate specificity between the bph and tod operons were investigated. The large and small subunits of the terminal dioxygease and the ring meta-cleavage compound hydrolase were critical for their discrete metabolic specificities. From the results obtained in this study it can be postulated that BP-utilizing bacteria, along with other aromatics utilizing bacteira, might be involved in the final degradation of plant lignin.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Furukawa,K.et al.: "Biochemical and genetic basis for the degrad-ation of polychlorinated biphenyls in soil bacteria" Pseudomonas:Molecular Biology and Biotechnology. 259-267 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Furukawa,K.: "Gene coding for PCB-degradation in soil bacteria" Now and Future. 8. 13-16 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Furukawa,K.et al.: "Gene components responsible for discrete substrate specificity in the catabolism of biphenyl(bph operon)and toluene(tod operon)" J.Bacteriol.175. 5224-5232 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Furukawa,K.et al.: "Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase" J.Bacteriol.(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Furukawa,K.et al.: "Construction of hybrid biphenyl(bph)and toluene(tod)genes for functional analysis of aromatic ring dioxygenases" Gene. 138. 27-33 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Furukawa,K.: "Molecular genetics and evolutionary relation-ship of PCB-degrading bacteria" Biodegradation. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Furukawa, S.Hayashida and K.Taira: "Biochemical and genetic basis for the degradation of polychlorinated biphenyls in soil bacteria" Pseudomonas : Molecular Biology and Biotechnology. 259-267 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Furukawa: "Genes coding for PCB-degradation in soil bacteria" Now and Future. 8. 13-16 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Furukawa, J.Hirose, A.Suyama, A.Zaili and S.Hayashida: "Gene components responsible for discrete substrate specificity in the catabolism of biphenyl (bph operon) and toluene (tod operon)" J.Bacteriol. 175. 5224-5232 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Furukawa, J.Hirose, S.Hayashida and K.Nakamura: "Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase" J.Bacteriol. 176(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] J.Hirose, A.Suyama, S.Hayashida and K.Fukrukawa: "Construction of hybrid biphenyl (bph) and toluene (tod) genes for functional analysis of aromatic ring dioxygenases" Gene. 138. 27-33 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Furukawa: "Molecular genetics and evolutionary relationship of PCB-degrading bacteria" Biodegradation. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K,Furukawa et.al.: "Gene components responsible fordiscrete substrate specifi-city in thecatabolism of biphenyl(bph operon)andtoluene (tod operon)" J,Bacteriol. 175. 5224-5232 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K,Furukawa et al.: "Efficient degradation of trichloroethylene by a hybrid aromotic ringdioxygenase" J,Bacteriol. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K,Furukawa et al.: "Construction of hybrid biphenyl(bph)and toluene (tod)genes for functional analysis of aromatic ring dioxygenases" Gene. 138. 27-33 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K,Furukawa et al.: "Molecular genetics and evolutionary relationship of PCB-degrading bacteria" Biodegradation. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Furukawa,K.et.al.: "Biochemical and genatic basis for the degradetion of polychlorinated biphenyls in soil bacteria" “Pseudomonas:Molecular Biology and Biotechnology". 259-267 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Furukawa,K.: "Genes coding for PCB-degradation in soil bacteria" Now and Future. 8. 13-16 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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