| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Research Abstract |
Carotenoids such as beta-carotene are considered to be effective inhibitors of lipid peroxidation. To know the mechanism of antioxidant activity of beta-carotene, it is important to analyze its oxidation products that may arise during its action as an antioxidant. In this research, the reaction products of beta-carotene with peroxyl radicals were isolated and characterized. beta-Carotene was reacted with an alkylperoxyl radical at 37ﾟC in benzene. 2,2'-Azobis(2,4-dimethylvaleronitrile) (AMVN) was used to generate the alkylperoxyl radical. The reaction products were identified as 12-formyl-11-nor-beta, beta-carotene
(1), 15'-formyl-15-nor-beta, beta-carotene
(2), 19-oxomethyl-10-nor-beta, beta-carotene
(3), 5,6-epoxy-5,6-dihydro-beta, beta-carotene
(4), 13,15'-epoxyvinyleno-13,15'-dihydro-beta, beta-carotene
(5), 15'13-epoxyvinyleno-13,15'-dihydro-beta, beta-carotene
(6), and 11,15'-cyclo-12,15-epoxy-11,12,15,15'-tetrahydro-beta, beta-carotene
(7). The reaction products of beta-carotene during the AMVN-induced peroxidation of methyl linoleate in bulk phase were also studied. In addition to compounds 1-7, a large amount of adducts of beta-carotene with methyl linoleate were formed during the peroxidation of methyl linoleate. The results indicate that beta-carotene can scavenge lipid-peroxyl radicals to form compounds 1-7 and adducts of beta-carotene with lipids.
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