Screening of Substances Masking the Activity of Attractants for Cerambycid Beetle, Monochamus Alternatus Hope, from its host.

• Project/Area Number: 04660146
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: 製造化学・食品
• Research Institution: Kagawa University
• Principal Investigator: YAMASAKI Toru Kagawa University, Faculty of Agriculture, Professor, 農学部, 教授 (50036042)
• Project Period (FY): 1992 – 1993
• Project Status: Completed (Fiscal Year 1993)
• Budget Amount: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 1993: ¥300,000 (Direct Cost: ¥300,000); Fiscal Year 1992: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: Monochamus alternatus / Cerambycid beetle / Pinus densiflora / Healthy pine / Attractants / Attractiveness-musking substances / Repose inducer / (+)‐juniperol / (+)‐pimaral / マスク物質 / 行動停止・静止作用

Research Abstract

(1) Terpene hydrocarbon fraction (H_T) of essential oil (E_T) from a trunk of Pinus desiflora masked (blocked/diminished) response by female cerambycid beetle, Monchamus alternatus to oxygenated terpene fraction (O_T) of E_T, with no instrict repellency.
(2) H_F fraction from foliage also masked the attractiveness of o_T, while O_F from foliage exhibited repellent activity.
(3) Crude masking substances X_T and X_F, respectively, were separated from residues (R_T, R_F) obtained by reduced-pressure distilllations of E_T and E_F, using TLC and GC.
(4) The crude X_T and X_F stopped the response by the female, and made her stand still. X_T and X_F were found to be busking substances.
(5) The substances were unstable ; sixty % of X_F in n-pentane changed into other compounds after 10 days.
(6) X_F was isolated from R_F by reversed phase HPLC.
(7) X_T and X_F will be isolated, biossayd, and chemically identified. Then, masking mechanism will be investigated.