| Project/Area Number |
04660147
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
製造化学・食品
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
MIYAMOTO Takahisa Kyushu Univ., Food Sci.& Technol., Assist.Prof., 農学部, 助手 (70190816)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIMOTO Makoto Kyushu Univ., Food Sci.& Technol., Assoc.Prof., 農学部, 助教授 (90182831)
HATANO Shoji Kyushu Univ., Food Sci.& Technol., Professor, 農学部, 教授 (30038260)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Bacillus cereus / Toxin / Vacuolation factor / Intestine 407 / HEp-2 / Detection / Intestine 407 細胞 / HE_P-2細胞 / 空砲化因子 |
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| Research Abstract |
Bacillus cereus strain isolated from causative food of food poisoning produced heat stable toxin causing morphological changes in culture cells of HEp-2 and Intestine 407. The toxin production was optium in rice and milk containing media. Although the tpoxin production was low in a semi-synthetic medium containing ammonium sulfate and yeast extract, the production increased by the addition of 0.5% glucose to the medium. Relationship between toxin production and aeration was investigated in the medium containing 0.5%glucose. With aeration by vigorous shaking, the toxin production was observed at 4th hr of cultivation and a maximal production was detected at 8th hr of cultivation. The spore was not detected at that time. The spore was detected at 24 hr. Without shaking, the rate of sporulation was very low even at 48hr though the extent of the toxin production was the same as that of the shaking culture. For purification of the toxin, salt-out with ammonium sulfate, n-butanol extraction and reversed-phase chromatography with C_<18> column were effective. Although the toxin was not purified completely, the toxin seems to contain a protein.
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