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Study on biotechnological production of biologically active substances from extracts of Japanese taxus trees.

Research Project

Project/Area Number 04660190
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 林産学
Research InstitutionEhime University

Principal Investigator

TACHIBANA Sanro  Ehime University, College of Agriculture, Associate Professor, 農学部, 助教授 (10112319)

Co-Investigator(Kenkyū-buntansha) OKI Tae  Ehime University, College of Agriculture, Associate Professor, 農学部, 助教授 (20116916)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsJapanese taxus trees / Kyaraboku / Tissue culture / Enzymatic conversion / Taxol / Taxine / Elicitor
Research Abstract

The aim of the study was biotechnological production of biologically active substances from extracts of Kyaraboku(Taxus cuspidata var.nana Rehd).
1. Extracts in leaves and bark of kyaraboku.
Two bisflavones (sciadopitysin and ginkgetin), two flavonols (isorhamnetin and quercetin), and two taxane-type of diterpenoids (taxinine and taxinine B) were isolated from the leaves of Kyaraboku. It was considered that taxinine could be converted to taxol mimic having anti-carcenogenic activity by enzymatic conversion with lipase.
From the bark of Kyaraboku, taxol having strong anti-carcenogenic activity, was isolated in the yield of 0.001% to dry bark. The content of taxol in the bark was very small, however, it was considered that content of taxol could be increased by tissue culture of Kyaraboku.
2.Pruduction of taxol mimic by enzymatic conversion of taxinine with lipase.
Taxol mimic can be produced by reduction of enone carbonyl group in taxinine and followed by esterfication with lipase. Taxol mimic's side chain, phenylisoserine was synthesized from E-cinnamic acid through 8 steps in the total yield of 15%. Reduction of the carbonyl group with di-butylaluminium hydride are now being conducted.
3. Production of taxol by tissue culture of Kyaraboku.
Taxol was found to be produced in the calli induced from Kyaraboku stems and leaves. The amount of taxol in the calli was 1.1 times higher than that in intact plant. To increase the amount of taxol, the calli was eliciated with chito-oligosaccharides in various concentrations. The amount of taxol was rised up to 10 times higher than that of untreated calli.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Sanro Tachibana: "Flavonoids in the leaves of Taxus brevifolia." Biochemical Biosystematic and Ecology. (投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sanro Tachibana: "Extractives in the leaves and bark of Taxus cuspidata var.nana Rehd." Mokuzai Gakkaishi. (投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sanro Tachibana: "Flavonoids in the leaves of Taxus brevifolia" Biochemical Bioststematics and Ecology. (to be published).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sanro Tachibana: "Extractives in the leaves and bark of Taxus cuspidata var.nana Rehd." Mokuzai gakkaishi. (to be published).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 橘 燦郎: "イチイ科樹木の組織培養(I) キャラボクの組織培養によるタキソールの生成" 第43回日本木材学会大会研究発表要旨集. 101 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 橘 燦郎: "イチイ科樹木の抽出成分(IV)-キャラボクの抽出成分並びに〓〓糸化合物合成のためのフェニルイソセリン側鎖部の合成-" 第43回日本木材学会大会研究発表要旨集. 343-343 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 松尾 敦: "イチイ科植物の抽出成文(II) キャラボク中のフラボノイドおよびタキサン系ジテルペンについて" 第42回日本木材学会大会研究発表要旨集. 426-426 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 松尾 敦: "イチイ科植物の抽出成分(III) タキソール系化合物の合成のためのフェニルイソセリンの側鎖部の合成" 日本木材学会 中国・四国支部 第4回研究発表会要旨集. 44-45 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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