Studies on Utilization of Marine Bacterial Functions Using Immobilized Cells
| Project/Area Number |
04660222
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | Mie University |
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Principal Investigator |
SUGAHARA Isao Mie Univ., Fac.Bioresources Professor, 生物資源学部, 教授 (80024826)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Toshio Mie Univ., Fac.Bioresources Assistant Researcher, 生物資源学部, 助手 (50024831)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | immobilized cells / bioremediation / marine bacterial functions / utilization of marine bacteria / convironment conservation / 細胞・固定化 / 海洋微生物機能 / 微生物の開発・利用 |
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| Research Abstract |
1. In order to utilize marine bacterial functions, an attempt to isolate useful microorganisms was made. Among 2687 strains of heterotrophic bacteria isolated, 37 strains of starch-decomposing bacteria, 13 strains of casein-decomposing bacteria and 1 strain of tributyrin-decomposer were selected. It was very difficult to isolate purely microalgae (oxygen generators) and ammonia-oxidizing bacteria. Among 897 strains of nitrogen-scavenging bacteria isolated, 5 strains having high ammonia-assimilating activity were selected.
2. The growht rate of nitrogen-scavenging bacteria increased with increasing in NH_4C1 concentration. The NH_4C1-affinity for growth of nitrogen-scavenging bacteria was very high, i.e. Ks value was 5.70 muM.
3. The optimum pH and temperature for ammonia uptake activity of kappa-carrageenan-immobilized cells of nitrogen-scavenging bacteria were pH 5-7 and 40ﾟC,respectively. The activity also increased with increasing in NH_4C1 concentration. The optimum pH and temperature for ammonia uptake activity of polyacrylamide gel-immobilized cells of nitrogen-scavenging bacteria were pH 7 and 30ﾟC,respectively. The efficientcy of ammonia removal from water was depended upon the amounts of immobilized cells gel.
4. In order to apply nitrogen-scavenging ability for complete removal of nitrogen in water, the model water treatment system was prepared. Nitrogen-scavenging bacteria rapidly decomposed organic matter and effectively removed ammonia from water. The ability to uptake NH_4^+ by immobilized cells was comparative to that by free suspended cells.
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Report
(4 results)
Research Products
(14 results)
