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Immunoelectron microscopy of vasotocin neurons in the chicken hypothalamu

Research Project

Project/Area Number 04660290
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionNagoya University

Principal Investigator

HIRUNAGI Kanjun  Nagoya Univ, School of Agricultual Science, Asst. Prof., 農学部, 助手 (00126898)

Co-Investigator(Kenkyū-buntansha) SAITO N.  Nagoya Univ, School of Agricultual Science, Asst. Prof., 農学部, 助手 (40211924)
NAITO J.  Nagoya Univ, School of Agricultual Science, Assoc. Prof., 農学部, 助教授 (30048467)
WATANABE T.  Nagoya Univ, School of Agricultual Science, Prof., 農学部, 教授 (30023427)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsChicken / Vasotocin neurons / Immunoelectron microscopy / Immunohisto chemistry / Hypathalamus / NPY / ニューロペプタイドY
Research Abstract

Vasotocin(VT)- immunoreactive(ir) neurons of the domestic chicken were studied by an immunohistochemical technique.
There are several VT-ir cell populations in the preoptic areas and periventricular regions of the hypothalamus. The sizes of these cell bodies are 22-25X15mum. Shape factors are 0.7-0.8. In preoptic areas VT-ir cell bodies are distributed just under a pia mater. In the periventricular region, there are many VT-ir processes and somata. Some of these immunoreactive fibers apparently originate in each of the immunoreactive somata in the subependymal zone. Thich immunoreactive processes running toward the third ventricle seem to reach the ependymal cell layr. These processes seem to be dendritic processes of immunoreactive neurons located in the subependymal region.
Immunoelectron microscopy of VT-ir neurons of the P-1 region(Berk et al. 1982)revealed VT-ir processes that make contact with the basal elements of ependymal cells of the third ventricle. On these VT-ir processes, immunonegative terminals are found. In the subependymal neuropil of the P-1 region, many immunoreactive processes of various sizes are found. Immunonegative synaptic terminals containing clear vesicles were frequently observed on thin immunopositive processes. The junctional comlex of ependymal cells seemes to lack a tight junction.
Immunoelectron microscopy of neuropeptide Y(NPY) revealed that there are many NPY-ir processes and terminal like formations in the P-1 region. At least two types of NPY-ir processes. First, some of precesses are axonal fibers. Second type of processes belongs to terminals. Terminal-like structures are 725-750nm in diameter. NPY-ir axonal terminals frequently contact with immunonegative dendrites in this region. These results suggest that VT-ir neurons of P-1 region receive a NPY projection. Further studies are needed to confirm the NPY projection of this region in the domestic chicken.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Hirunagi et al.: "Immunocytochemical demonstration of serotonin-immunoreactive cerebrospinal fluid-contacting neurons in the paraventricular organ of pigeons and domestic chickens." Progress in Brain Res.91. 327-330 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirunagi et al.: "Electron microscopic inrestigations of vasoactive intestinal peptide(VIP)-like immunoreactive terminal formations in the lateral septum of the pigeon(Columba livia)." Cell Tissue Res.(1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naito et al.: "The spinal segmental and preganglionic projections from the brain stem in relation to the chicken cloaca." J.Hirnforsch. (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirunagi et al.: "Immunocytochemical demonstration of vasotocin (AVT) and mesotocin (MT) neurons in the domestic chicken" Proceeding of the Japan Society for Comparative Endocrinology. 6. 52 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Korf et al.: "Cerebrospinal fluid-contacting neurons:morphological and physiological considerations" In:Integrative and Cellular Aspects of Autonomic Functions:temperature-and osmo regulation Libbey,Paris.407-418 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Saito et al.: "Ovarian prostaglandin and arginin vasotocin in relation to oviposition" In:Avian Endocrinology,Journal of Endocrinology,Bristol.359-366 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirunagi, K., Hasegawa, M., Vigh B.& Vigh-Teichmann, I.: "Immunocytochemical demonstration of serotonin-immunoreactive cerebrospinal fluid-contacting neurons in the paraventricular organ of pigeons and domestic chickens" Progress in Brain Research. 91. 327-330 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirunagi, K.: "Immunocytochemical demonstration of vasoactive intestinal polypeptide(VIP)-immunoreactive cerebrospinal fluid(CSF)-contacting neurons in the lateral septum of the duck" Neuroscience Research. Suppl.18, S98. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirunagi, K., Kiyoshi, K., Adachi, A., Hasegawa, M., Ebihara, S.& KorfH.-W.: "Electron microscopic investigations of vasoactive intestinal peptide(VIP)-like immunoreactive terminal formations in the lateral septum of the pigeon(Columba livia)." Cell Tissue Res. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naito, J., Ohmori, Y., Kimura, N., Shiraishi, M., & Watanabe, T.: "The spinal segmental and preganglionic projections from the brainstem in relation ro the chicken cloaca." J Hirnforsch. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirunagi, k., Nonoyama, K., Saito, N.& Koike, T.I.: "Immunocytochemical demonstration of vasotocin(AVT)and mesotocin(MT)neurons in the domestic chicken" Proceedings of the Japan Society for Comparative Endocrinology No.6, 1991(Hirano, T.& Takei, Y.eds.). Japan Society for Comparative Endocrinology, Tokyo. 52

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Korf, H.-W., Rommel, E., Hirunagi, K.& Oksche, A.: "Cerebrospinal fluid-contacting neurons : morphological and physiological consideration" Pleschka, K., Gerstberger, R.(eds)Integrative and Cellular Aspects of Autonomic Functions : temperature- and osmoregulation Libbey, Paris. 407-418 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Saito, N., Shimada, K., & Koike T.I.: "Ovarian pros taglandin and arginin vasotocin in relation to oviposition" Sharp, P.J.(ed)Avian Endocrinology, Journal of Endocrinology, Bristol. 356-366 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naito J.et al.: "The spinal segmental and preganglionic projections from the brain stem in relation to the chicken cloaca" J.fur Hirnforsung.(1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hirunagi K.et al.: "Electron microscopic investigations Vasoactive Intestinal Peptide (VIP)-like immunoreactive terminal formations in the lateral septum." Cell.Tissue Res.(1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hirunagi et al.: "Immunocy to chemical demonstration of vasotocin(AVT) and mesotocin(MT) neurons in the domestic chicken." Proceedings of the Japan Society for comparative endocrinology.6. 52- (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Saito et al.: "Ovarian prostaglandin and arginine vasotocin in relation to oviposition" V International Symposium on Avian Endcrinology. Programme and Abstracts Edinburgh. Scotland 13th-17th September 1992. 45- (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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