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The role of plasmalemmal Ca^<2+> channels and intracellular Ca^<2+> stores in vascular smooth muscles during the development of vascular resistance

Research Project

Project/Area Number 04660325
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionMiyazaki University

Principal Investigator

ITO Katsuaki  Miyazaki University, Faculty of Agriculture, Professor, 農学部, 教授 (70136795)

Co-Investigator(Kenkyū-buntansha) 村上 和保  宮崎大学, 農学部, 助教授 (00190877)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsResistance vessels / Ca channels / Sarcoplasmic reticulum / Contraction / Intracellular Ca / Contractile protein / Phosphorylation / Membrane potential / 血管平骨筋 / ryanodine
Research Abstract

In this study, the roles of plasmalemmal Ca^<2+> channels and sarcoplasmic reticulum (SR), intracellular Ca^<2+> stores, in the development of tension in vascular smooth muscle, especially in resistance vessels, were investigated. The major findings are as follws.
1) Regulation of cytoplasmic Ca^<2+> ([Ca^<2+>]i) by Ca^<2+> channel and SR.I analyzed the effects of ryanodine and cyclopiazonic acid, modifiers of SR functions, on [Ca^<2+>]i, mesured with fura-2, and the tension of rat mesenteric resistance arteries. The results suggest that SR plays as a buffer to decrease [Ca^<2+>]i at a resting state, while it amplifies a contraction by releasing Ca^<2+> when transmembrane Ca^<2+> influx increased.
2) Ca^<2+> entry pathways following activation of alpha1-adrenoceptor. When alpha1-adrenoceptors of resistance vassels was activated by low concentration of agonist, Ca^<2+> influx through voltage-dependent Ca^<2+> channel (VDC) was enhanced without any depolarization. This influx triggered Ca^<2+> release from SR.A high concentration of agonist caused depolarization, which in turn enhanced the Ca^<2+> influx through VDC.Besides this, Ca^<2+> entry through a pathway other than VDC also occurred. This entry was sensitive to K^+ channel blockers but not to dihydropyridine Ca^<2+> channel blockers.
3) Functions of ion channels in the hypertensive vessels. In vascular smooth muscles from spontaneously hypertensive rate voltage-dependent Ca^<2+> channels were active at the resting state, which produced an active tension and activated Ca^<2+>-activated K^+ channels. Increased Ca^<2+> influx provided more Ca^<2+> to SR, then increased Ca^<2+> release from SR.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Asano,M.,Masuzawa-Ito,K.,Matsuda,T.,Imaizumi,Y.,Watanabe,M.& Ito,K.: "Functional role of Ca^<2+>-activated K^+ channels in resting state of carotid arteries from SHR" Am.J.Physiol.265. H843-H851 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Asano,M.,Matsuda,T.,Hayakawa,M.,Ito,K.M.& Ito,K.: "Increased resting Ca^<2+> maintains the myogenic K^+ channels in arteries from young spontaneously hypertensive rats" Eur.J.Pharmacol.Mol.Pharmacol.247. 295-304 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naganobu,K.& Ito,K.: "Handling of cytoplasmic Ca^<2+> by sarcoplasmic reticulum during α_1-adrenoceptor mediated contraction of rat mesenteric resistance arteries" Jpn.J.Pharmacol.64. 89-96 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naganobu,K.,Takagi,M.,Kawasaki,H.& Ito,K.: "Modification by cyclopiazonic acid and ryanodine of depolarization-induced constriction in rat mesenteric artery" Eur.J.Pharmacol.251. 307-310 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 永延清和、伊藤勝昭(浦川紀元、唐木英明 編): "平滑筋実験法入門(V.抵抗血管の機械的反応測定法を分担執筆)" 文永堂出版(印刷中), (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Asano, M., Masuzawa-Ito, K., Matsuda, T., Imaizumi, Y., Watanabe, M.&Ito, K.: "Functional role of Ca^<2+>-activated K^+ channels in resting state of carotid arteries from SHR." American Journal Physiology. 265. H843-H851 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Asano, M., Matsuda, T., Hayakawa, M., Ito, K.M.&Ito, K.: "Increased resting Ca^<2+> maintains the myogenic tone and activated K^+ channels in arteries from young spontaneously hypertensive rats." European Journal of Molecular Pharmacolpgy. 247. 295-304 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naganobu, K.&Ito, K.: "Handling of cytoplasmic Ca^<2+> by sarcoplasmic reticulum during alpha1-adrenoceptor mediated contraction of rat mesenteric resistance arteries." Japanese Journal of Pharmacology. 64. 89-96 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naganobu, K., Takagi, M., Kawasaki, H.&Ito, K.: "Modification by cyclopiazonic acid and ryanodine of depolarization-induced constriction in rat mesenteric artery." European Journal of Pharmacology. 251. 307-310 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Naganobu, K.&Ito, K.: Methods to measure mechanical response of small arteries. (in Japanese). Introduction to Experiments on Smooth Muscles (ed.Urakawa, N.&Karaki, H.) Bun'eido Syuppan (in press),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Asano,M.,Masuzawa-Ito,K.Matsuda,T.,Imaizumi,Y.,Watanabe,M.& Ito,K.: "Functional role of Ca^<2+>-activated K^+ channels in resting state of carotid arteries from SHR" Am.J.Physiol.265. H843-H851 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Asano,M., Matsuda,T., Hayakawa,M., Ito,K.M. & Ito,K.: "Increased resting Ca^<2+> maintains the myogenic K^+ channels in arteries from young spontaneously hypertensive rats" Eur.J.Pharmacol.Mol.Pharmacol.247. 295-304 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Naganobu,K. & Ito,K.: "Handling of cytoplasmic Ca^<2+> by sarcoplasmic reticulum during alpha1-adrenoceptor mediated contraction of rat mesenteric resistance arteries" Jpn.J.Pharmacol.64. 89-96 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Naganobu,K., Takagi,M., Kawasaki,H. & Ito,K.: "Modification by cyclopiazonic acid and ryanodine of depolarization-induced constriction in rat mesenteric artery" Eur.J.Pharmacol.251. 307-310 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 永延清和、伊藤勝昭、(浦川紀元、唐木英明 編): "平滑筋実験法入門 (V.抵抗血管の機械的反応測定法を分担執筆)" 文永堂出版,(印刷中), (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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