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MECHANISM OF CELL DIFFERENTIATION OF ENDOCRINE CELL, WITH SPECIAL REFERENCE TO THE SOMATOMAMMOTROPH IN ANTERIOR PITUITARY GLAND.

Research Project

Project/Area Number 04670003
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 解剖学一般(含組織学・発生学)
Research InstitutionFACULTY OF SCIENCE OF SAITAMA UNIVERSITY (1993)
Gunma University (1992)

Principal Investigator

INOUE Kinji  SAITAMA UNIV., SCIENCE, PROFESSOR, 理学部, 教授 (50091963)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
KeywordsPITUITARY GLAND / GROWTH HORMONE CELL / PROLACTIN CELL / SOMATOMAMMOTROPH / INSULIN / INSULIN LIKE GROWTH FACTOR / CELL PROLIFERATION / TRANSDIFFERENCIATION / 下垂体前葉 / 成長ホルモン / プロラクチン / 3胞星状細胞
Research Abstract

The endocrine gland, such as an anterior gland consist from the mixed population of different hormone secreting glandular cells. These different glandular cells supposedly differentiated from same stem cell however, the mechanism of cell differentiation in endocrine cells still under discusson. We recently established cell line(MtT/S)from rat plaractinoma. The MtT/S cell line produces only growth hormone(GH)in normal growth medium. The MtT/S cells labeled with bromodeoxyuridine(BrdU)also show staining for GH.Furthermore, mitotic MtT/S cells contain GH immunopositive secretory granules in their cytoplasm. These features indicate that MtT/D cells proliferate as GH-secreting cells. We reported previouly that MtT/S cells transdifferentiate into prolactin cells upon stimulation with insulin or insulin-like growth factor. A requirement of estrogen priming for MtT/S cell transdifferentiation has also been reported. This transdifferentiation is considerably accelerated by addition of EGF, resulting in somatomammotrophs contain both prolactin and GH.In order to reveal the relationship between cell proliferation and cell differentiation, a BrdU labeling experiment was performed. the results indicated that prolactin cells which were induced by stimulation with insulin and EGF showed markedly suppressed BrdU incorporation, indicating that prolactin cells that have transdifferentiated from growth hormone cells are non-proliferate or show only very slow proliferation. In general, prolactin cells are known to be the most active proliferative cells in the pituitary gland. This discrepancy between in vitro and in vivo result is verly important, since it may indicate an unknown mechanism for the regulation of prolacrtin cell proliferation in the anterior pituitary gland. On the other hand these cell differentiation may be controlled by neighboring supportive cells through cell cell communication. The folliculo-stellate cells in the anterior pituitary gland is the most important cand

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Sakai T.et.al.: "Light and Electron microscopic immunocytochmistry of TSH-like cells occuring in the pars tuberalis of the adult male rat pituiaty" Ach.Histol.Cytol.55. 151-157 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] "Development of prolactin and growth hormone production in the fetal rat pituitary:an immunochemical study" Dev.Growth Differ.34. 473-478 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nagata et.al: "Incleased peptidylarginine deiminase expression during induction of prolactin biosynthesis in a growth-hormone-producing rat pituitary cell line,MtT/S." J.Cell.Physiol.15. 426-432 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue K.et.al.: "Establishment of a folliculo-stellate-like cell line from a murine thyrotropic pituitary tumor" Endocrinology. 131. 3110-3115 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yasuda H.et al.: "Existence of activin A in A-and D-cells of rat pancreatic islet." Endocrinology. 133. 624-630 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto H.et.al.: "Newly established murine pituitary folliculo-stellate-like cell line(TtT/GF)secretes potent pituitary glandular cell survival factors,one of which corresponds to metallopretinase inhibitor." Biochem.Biophys.Res.Comm.194. 909-915 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Akiyama K.et al.: "Search for functional significance of peptidyolarginine deiminaswe in rat pituitaries:Variation during pregnancy and ultrastructural localization in prolactin cells" Cell Biolol.International.17. 487-494 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] kato et.al.: "In Situ binding for detection of proteins that bind to regulatory element." Biochem.Biophys.Res.Comm.195. 963-968 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sakai T., Inoue K.and Kurosumi K.: "Light and Electron microscopic immunocytochemistry of TSH-like cells occuring in the pars tuberalis of the adult male rat pituitary." Arh.Histol.Cytol.55. 151-157 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nagata S., Rosenfeld M.G.and Inoue K.: "Development of prolactin and growth hormone production in the fetal rat pituitary : an immunochemical study." Dev.Growth Differ. 34. 473-478 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Nagata S., Uehara T., Inoue K.and Senshu T.: "Increased peptidylarginine deiminase expression during induction of prolactin biosynthesis in a growth-hormone-producing rat pituitary cell line, MtT/S." J.Cell.Physiol. 150. 426-432 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue K., Matsumoto H., koyama C., Shibata K., Nakazato Y.and Ito A.: "Establishment of a folliculo-stellate-like cell line from a murine thyrotropic pituitary tumor." Endocrinology. 131. 3110-3115 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yasuda H., Inoue K., Shibata H., Takeuchi T., Eto Y., Hasegawa Y., Sekine N., Totsuka Y., Mine T., Ogata E.and Kojima I.: "Existence of activin A in A- and D- cells of rat pancreatic islet." Endocrinology. 133. 624-630 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto H., Ishibashi Y., Ohtani T., Hasegawa Y., Koyama C.and K.: "Newly establishied murine pituitary folliculo-stellate-like cell line(TtT/GF)secretes potent pituitary glandular cell survival factors, one of which corresponds to metallopoteinase inhibitor." Biochem.Biophys.Res.Comm.194. 909-915 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Akiyama K., Nagata S., Tanaka Sh., Inoue K., Watanebe K.and Senshu T.: "search for functional siginificance of peptidylarginine deiminase in rat pituitaries : Variation during pregnancy and ultrastructural localization in prolactin cells." Cell Bilol.International. Vol.17. 487-494 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kato Y., Kato T., Ezashi T.and Inoue K.: "In Situ Binding for detection of proteins that bind to regulatiory elements." Biochem.Biophys.Res.Comm.195. 963-968 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto H., Koyama C., Sawada T., Koike K., Hirota K., Miyake A., Arimura A.and Inoue K.: "Pituitary folliculo-stellate-like cell line(TtT/GF)responds to novel hypophysiotropic peptide(pituitary adenylate cyclase-activating peptide), showing increased adenosine 3', 5'-monophosphate and interleukin-6 secretion and cell proliferation." Endocrinology. 133. 2150-2155

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mitsuishi T., Akuzawa Y., Sato S., Rui J., Kodama K., Inoue K., Kurashige S.: "Immunological properties of TtT/M-87 cell line established from murine pituitary tumor-associated macrophages." Microbiol.Immunol.37(12). 943-951 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matsumoto H.et.al.: "Pituitary folliculo-stellate-like cell line(TtT/GF)responds to novel hypophysiotropic peptide(pititary adenylate cyclase-activating peptide), showing increased adenosine 3', 5 '-monophosphate and interleukin-6 secretion and cell proliferation." Endocrinology. 133. 2150-2155 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Mirtsuishi T.et.al.: "Immunological properties of TtT/M-87 cell line established from murine pituitary tumor-assosiate macrophages." Microbilol.Immunol.37. 943-951 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Matumoto H.et al.: "Pitu9itary folliculo-stellate-like cell line(TtT/GF)responds to novel hypophysiotropic peptide(pituitary adenylate cyclase-activating peptide),showing increased adenosine 3,5-monophosphate and interleukin-6 secretion and cell proliferation." Endocrinology. 133. 2150-2155 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kato Y.et al.: "In Situ Binding for detection of proteins that bind to regulatory elements." Biochem.Biophys.Res.Comm.195. 963-968 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Matsumoto H.et al.: "Newly estabsished murine pituitary folliculo-stellate-like cell line(TtT/GF)secretes potent pituitary glandular cell survival factors,one of which corresponds to metalloproteinase inhibitor." Biochem.Biophys.Res.Comm.194. 909-915 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yasuda H.et al.: "Existence of activin A in A- and D-cells of rat pancreatic islet." Endocrinology. 133. 624-630 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 井上金治: "下垂体成長ホルモン産生細胞のプロラクチン産生細胞への分化転換" 脳神経外科速報. 3. 839-842 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 井上金治、藤田恒夫: "下垂体前葉細胞" 新薬と治療. 43. 20-22 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sakai et al.: "Light and electnon micnoscopic immunocyto chewistny of TSH-like cells occuning in the pans tuberalis of the adult malenat pitaitany" Arch.Histol,Cytal,. 55. 151-157 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nagata et al,: "Devolopment of pnolactin and gnowth hsomone production in the fetal not pituitany:an imomunochemieal study" Dw,Growth.Diffen,. 34. 473-478 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Inoue et al,: "Establishment of a folliculo-stellate-like coll line from a murine thynotnopic pituitany tumor" Euolocrinology. 131. 3110-3115 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 井上 金治: "ラット:GH_3" 生体の科学. 43. 422- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 井上 金治: "ラット:MATIS" 生体の科学. 43. 423- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 井上 金治: "マウス:TATIM-87" 生体の科学. 43. 483- (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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