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Molecular biological study on the melatonin synthesis during the transformation from photoreceptor cells to endocrine cells

Research Project

Project/Area Number 04670007
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SATO Tetsuji  School of Medicine, Deprtment of Anatomy, Lecturer, 医学部, 講師 (10162447)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsPineal organ / Heterogeneity / Photoreceptor cell / Endocrine cell / Hydroxyindole O-methyltransferase / Immunohistochemistry / In situ hybridization / Confocal laser microscopy / 松果体細胞 / 網膜 / 光受容関連タンパク / 神経特異エノラーゼ / メラトニン合成 / ヒドロキシインドールO-メチルトランスフェラーゼ
Research Abstract

Homogeneity within populations of mammalian pinealocytes has long been discussed. Several pineal studies have shown the variable localization of hydroxyindole O-methyltransferase(HIOMT), retinal S-antigen, and neuron-specific enolase(NSE). However, in the previous studies describing morphological heterogeneity, no stecific distributions of cell groups are mentioned, with the exception of variations among neighboring pinealocytes.
In the present study, we demonstrated a cortical and medullary arrangement of parenchymal cells in the bovine pineal gland by using antibodies for NSE, synaptophysin(SY-38), and HIOMT as markers of pinealocytes, and glial fibrillary acidic protein(GFAP) as a marker of interstitial cells. Immunoreactive pinealocytes of each antigen examined were more densely distributed in the cortex than in the medulla. In the cortex, GFAP-positive interstitial cells had large intensely-stained soma endowed with several long cytoplasmic processes, whereas in the medulla, they smaller, less intensely labeled perikarya from which numbers of fine short processes emerged. Furthermore, by means of probes specific for HIOMT mRNA, we examined possible differences in melatonin metabolic activity between the cortex and the medulla. An analysis using confocal laser microscopy together with in situ hybridization for HIOMT mRNA showed that the expression of mRNA transcripts in the cortex was more intense than that in the medulla. Our results suggest that the pinealocytes in the cortex are more secretory-like elements than those in the medulla.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Sato T,Kaneko M,Fujieda H,Deguchi T,Wake K: "Analysis of the heterogeneity within bovine pineal gland by immunohistochemistry and in situ hybridization" Cell Tissue Res. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujieda H,Sato T,Wake K,Takaoka M,Morimoto S: "Tissue kallikrein in the rat pineal gland:An immunocytochemical study" J.Pineal Res.14. 84-88 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kusakari T,Sato T,Yoshino Y,Funata M,Tokoro T: "Possible mechanism of the scleral changes in chicks with visual deprivation myopia" Invest Ophthalmol Vis Sci. 34. 879 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sato T,Fujieda H,Kaneko M,Wake K: "Expression of neuron-specific proteins(NSE,SY38)during the development of the rat pineal" Acta Anatomica Nipponica. 68. 661 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujieda H,Sato T,Wake K: "An immunocytochemical analysis for neuron-specific enolase(NSE)and synaptophysin(SY38)in the developing rat retina." Acta Anatomica Nipponica. 68. 688 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kaneko M,Sato T,Wake K: "Post-hatching development of glial fibrillary acidic protein(GFA)-immunoreactive cells in the pineal organ of the domestic fowl." Acta Anatomica Nipponica. 68. 661 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sato T,Wake K: "Atlas of endocrine organs" Springer-Verlag, 10 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 佐藤 哲二: "間脳・下垂体疾患(最新内科学体系12)" 中山書店, 6 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sato T, Kaneko M, Fjieda H, Deguchi T, Wake K: "Analysis of the heterogeneity within bovine pineal gland by immunohistochemistry and in situ hybridization" Cell Tissue Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fjieda H, Sato T, Wake K, Takaoka M, Morimoto S: "Tissue kallikrein in the rat pineal gland : An immunocytochemical study" J.Pineal Res.14. 84-88 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kusakari T, Sato T, Yoshino Y, Funata M, Tokoro T: "Possible mechanism of the scleral changes in chicks with visual deprivation myopia" Invest.Ophthalmol.Vis.Sci.34. 879 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sato T, Fjieda H, Kaneko M, Wake K: "Expression of neuron-specific proteins(NSE, SY38) during the development of the rat pineal gland" Acta Anatomica Nipponica. 68. 661 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Fujieda H, Sato T, Wake K: "An immunocytochemical analysis for neuron-specific enolase(NSE) and synaptophysin(SY38) in the developing rat retina" Acta Anatomica Nipponica. 68. 688 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kaneko M, Sato T, Wake K: "Post-hatching development of glial fibrillary acidic protein(GFA)-immunoreactive cells in the pineal organ of the domestic fowl" Acta Anatomica Nipponica. 68. 661 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sato T, Wake K: "Pineal organ" Atlas of endocrine organs.Springer-Verlag. 11-24 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sato T: "Diseases of the diencephalon and the hypophysis" Saishin-Naikagaku-Taikei 12 Nakayama-Shoten. 145-150 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Sato T,Kaneko M,Fujieda H,Deguchi T,Wake K: "Analysis of the heterogeneity within bovine pineal gland by immunohistochemistry and in situ hybridization" Cell Tissue Res. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Fujieda H,Sato T,Wake K,Takaoka M,Morimoto S: "Tissue kallikrein in the rat pineal gland:An immunocytochemical study" J.Pineal Res.14. 84-88 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kusakari T,Sato T,Yoshino Y,Funata M,Tokoro T: "Possible mechanism of the scleral changes in chicks with visual deprivation myopia" Invest Ophthalmol Vis Sci. 34. 879 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sato T,Kaneko M,Fujieda H,Wake K: "Zonal heterogeneity in the bovine pineal gland:analysis by using of immunocytochemistry and in situ hybridization" Acta Anat Nippon. 67. 492 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fujieda M,Sato T,Wake K: "Neuron-specific enolase in the developing rat pineal organ:An immunocytochemical study" Acta Anat Nippon. 67. 492 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kaneko M,Sato T,Wake K: "Immunohistochemical localization of hydroxyindole O-methyltransferase(HIOMT)in the chick pineal organ during post-hatching development" Acta Anat Nippon. 67. 565 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sato T,Wake K: "Atlas of endocrine organs" Springer-Verlag, 10 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 佐藤 哲二: "間脳・下垂体疾患(最新内〓学体系12)" 中山書店, 6 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 佐藤 哲二: "光受容関連タンパクの発現からみたニワトリの松果体と網膜" 解剖学雑誌. 67. 274- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 金子 道成,佐藤 哲二,ウィチャイ・エカタクシン: "ニワトリ松果体細胞の孵化後発達とneuron-specific enolase" 解剖学雑誌. 67. 274- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Fujieda H,Sato T,Wake K: "Neuron-specific enolase in the developing rat pineal organ:An immunocytochemical study" 解剖学雑誌. 67. 492- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sato T,Kaneko M,Fujieda H,Wake K: "Zonal heterogeneity in the bovine pineal gland:Analysis by using immunocytochemistry and in situ hybridization" 解剖学雑誌. 67. 492- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kaneko M,Sato T,Wake K: "Immunocytochemical localization of hydroxyindole O-methyltransferase (HIOMT) in the chick pineal organ during post-hatching development" 解剖学雑誌. 67. 565- (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Fujieda H,Sato T,Wake K,Takaoka M,Morimoto S: "Tissue kallikrein in the rat pineal gland-An immunocytochemical study" Journal of Pineal Research.

    • Related Report
      1992 Annual Research Report
  • [Publications] Sato T & Wake K: "Atlas of Endocrine Organs-Vertebrates and Invertebrates" Springer-Verlag, 307 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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