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The role of the notochord to the morphogenesis of chick embryos with special reference to the differentiation of somites and limbs

Research Project

Project/Area Number 04670008
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

HIRANO Shigeki  Niigata Univ., Sch.Med., Dept.Anatomy, Associate Prof., 医学部, 助教授 (10018765)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsNOTOCHORD / SOMITE / SCLEROTOME / MYOTOME / NEURAL TUBE / CHICK / DERMOMYOTOME / 沿軸中胚葉 / 肢芽 / 分化
Research Abstract

In order to determine the developmental relationships among the notochord, somites, and limbs, various micro-surgical manipulations were performed on chick embryos. When the notochord was transplanted onto the dorsal midline of the neural tube, the majority of the embryos developed without an abdominal wall and thus had exposed internal organs. In addition, their limbs grew toward the dorsal aspects of the embryo, as if the dorso-ventral orientation of the embryo had been changed. All such experimentally manipulated embryos were dead before embryonic day 10. Because transplantation of the notochord had such a deleterious effect on morphogenesis, it was impossible to determine the relationship between the notochord and limb development directly. Before we could address this issue, we firsts had to understand the developmental relationship between the notochord and somites. When somites were deprived from the influences of both the neural tube and notochord, all of the cells that compris … More e the somites were dead within 10 hours of operation ; after 24 hours, neither dermomyotomes nor sclerotomes were observed adjacent to the neural tube. However, when the somites were deprived of the notochord, the sclerotome cells had died within 24 hours, leaving a void surrounded by the dermomyotomes. The vertebral columns and ribs did not develop on the operated side because they derived from sclerotomes. Though the arrangement of muscle cells was quite irregular, however, they developed above the mesonephros by day 7. In contrast, when only the neural tube was eliminated, small dermomyotomes appeared distal to the neural tube ; these ultimately differentiated to small clusters of muscle cells.
These results indicated that myotomes development requires influences from either neural tube or notochord, and sclerotome development requires only influences from the notochord. More data is required before the developmental relationship between the myotomes and the neural tube and notochord can be fully appreciated. Less

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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