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Relation between rat early ontogenical development of hemopoietic focus and placenta.

Research Project

Project/Area Number 04670018
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionYamaguchi University

Principal Investigator

FUJIKURA Yoshihisa  Yamaguchi University School of Medicine, First Department of Anatomy, Associate Professor, 医学部, 助教授 (10165368)

Co-Investigator(Kenkyū-buntansha) TOKUDA Nobuko  Yamaguchi University School of Medicine, First Department of Anatomy, Research A, 医学部, 助手 (70227578)
SAWADA Tomoo  Yamaguchi University School of Medicine, First Department of Anatomy, Associate, 医学部, 講師 (90187295)
FUKUMOTO Tetsuo  Yamaguchi University School of Medicine, First Department of Anatomy, Professor, 医学部, 教授 (00040171)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsRat / Hemopoietic region / Hemopoietic cell / Placenta / Immunohistochemistry / Monoclonal antibody / Culture / 造血細胞
Research Abstract

This research was done to clear where main hemopoiesis occurs during rat ontogenical development from yolk sac, fetal liver, fetal or neonatal spleen to bone marrow and also examined the organ, cell or factor which support the hemopoiesis. It is mentioned that hemopoietic cell, stromal cell and hemopoietic stimulating factor, are necessary to achieve hemopoiesis. We have reported UB-12 monoclonal antibody which was raised against 15days fetal liver cells, mainly hemopoietic cells, recognized rat hemopoietic cells of early stage. On the other hand, culture supernatant of placenta is usually used as a source of hemopoietic stimulating factor in vitro assay (CFU-C) . In this study, we analyzed the relation between hemopoietic stromal cells and stimulating factor.
We showed that UB-12 antigen was expressed on placenta tissue by immunofluorescence method. It has been suggested that placenta plays an important role in hemopoiesis of rat. We tried to make a cell line from rat placentatissue, b … More ut could not obtain. We isografted dissociated hepatocytes which were prepared from collagenase perfused rat liver to spleen, salivary gland, thymus or subcapsular region of kidney of partially hepatectomized rats. The transplanted hepatocytes formed liver tissue heterotopically, which had 3 faces of hepatocytes, such as sinusoidal, bile-canalicular and contiguous faces. The results were examined by using HAM2, HAM4 and HAM8 monoclonal antibodies. When dissociated hepatocytes from fetal rat liver were transplanted heterotopically, as same as above mentioned methods, they form not only liver tissue but also duct-like structure in situ. The results suggested that fetal hepatocytes are more undifferentiated than adult rat hepatocytes. We made new monoclonal antibodies which were named UB18 and UB19, against 18-day fetal rat liver cells. These antibodies reacted to adult rat hepatocytes but not fetal rat hepatocytes. This may mean both hepatocytes expressed different cell surface antigens on each cell, and we consider that fetal rat hepatocyte is one of the candidates of hemopoietic stromal cells in rat. Less

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Sawada,T.et al.: "Distrubution of the surface antigen HAM-4 and cytoskeleton during reformation of bile-canalicular structures in rat primary cultured hepatocytes" Exp.Cell Res.199. 50-55 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hirozane,A.et al.: "Analysis of MHC Class I antigen in rat salivary gland" Arch.Oral Biol.37. 147-151 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takai,K.et al.: "Effects of FK506 on rat thymic epithelial cells;Immunohistochemical study" Thymus. 19. 207-217 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujikura,Y.et al.: "Immunohistochemical analysis of rat liver using a monoclonal antibody (HAM8) against gap junction" Anat.Rec.235. 335-341 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Miyakawa,I.et al: "Preparation of a monoclonal antibody specific for a 48-kDa protein from mitochondrial uncleoids of the yeast,Saccharomyces cerevisiae" Plant.Cell Physiol.34. 151-156 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 副島京子 他: "実験的放射線肺臓炎モデルにおけるマクロファージ表面抗原の検討" リンパ学. 16. 35-40 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujikura,Y.et al.: "Immunohistochemical characterization of transplatable rat squamous cell carcinoma(FF6) in skin and thymus" Histol.Histopathol.8. 637-644 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakamura,K.et al.: "Affinity maturation of anti-hapten antibodies in a single mouse analyzed by two-dimensional affinity electrophoresis" Elpho.14. 1338-1340 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujikura,Y.et al.: "Membrane antigen expression of syngenically but heterotopically transplanted hepatocytes in rats" Cell Transplant.3. 23-31 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsuchida,M.et al.: "Analysis of cell surface antigen on glucocorticoid-treated rat thymocytes with monoclonal antibodies" Immunol.Lett.39. 209-217 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsuchida,M.et al.: "The effects of FK506 and dexamethasone on rat thymocyte differentiation" Therapeutic Immunol.1. 135-141 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsuchida,M.et al.: "Effects of irradiation,glucocorticoid,and FK506 on cell surface antigen expression by rat thymocytes:a three-color flow cytoflurometric analysis" Immunology. 83. 469-475 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Konishi,M.et al.: "Effects of FK506 on surface antigen expression by regenerating thymocytes after sublethal irradiation in the rat" Thymus. 23. 53-66 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tokuda,N.et al.: "Changes in the distrubution and intensity of alkaline phosphatase activity in rat lymph nodes and spleen cells after antigen stimulation" Acta Anat.151. 54-61 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fukumoto,T.et al.: "Analysis of cell surface antigens using monoclonal antibodies against rat hepatocyte,paticularly using HAM1" Exp.Mol.Pathol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kuniki,H.et al.: "Immunohistochemical localization and biological significance of the phylogenically conserved thymus-brain antigen(UB-13) in skate,rat and human" Vet.Immunol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujikura,Y.et al.: "Gap junction" Elsevier Science,Amsterdam,Netherland Edited by Kanno,Y., 4 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Fujikura, H.Kuniki, T.Sawada, K.Hamano, T.Akino, M.Shigetomi, A.Hirozane and T.Fukumoto: "Membrane antigen expression of syngenically but heterotopically transplanted hepatocytes in rats." Cell Transplant.3 (1). 23-31 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Tsuchida, M.Konishi, K.Jojima, K.Naito, Y.Fujikura and T.Fukumoto: "Analysis of cell surface antigen on glucocorticoid-treated rat thymocytes with monoclonal antibodies." Immunol. Lett.39. 209-217 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Tsuchida, K.Takai, K.Jojima, K.Naito, Y.Fujikura and T.Fukumoto: "The effects of FK506 and dexamethasone on rat thymocyte differentiation." Therapeutic Immunol.1. 135-141 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Tsuchida, M.Konishi, K.Takai, K.Naito, Y.Fujikura and T.Fukumoto: "Effects of irradiation, glucocorticoid, and FK506 on cell surface antigen expression by rat thymocytes : a three-color flow cytofluorometric analysis." Immunology. 83. 469-475 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Konishi, K.Takai, K.Jojima, Y.Fujikura, K.Naito and T.Fukumoto: "Effects of FK506 on surface antigen expression by regenerating thymocytes after sublethal irradiation in the rat." Thymus. 23. 53-66 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N.Tokuda, Y.Fujikura, T.Sawada, Y.Ohba and T.Fukumoto: "Changes in the distribution and intensity of alkaline phosphatase activity in rat lymph nodes and spleen cells after antigen stimulation." Acta Anat.151. 54-61 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Fukumoto, K.Tamakoshi, Y.Fujikura, T.Sawada, N.Tokuda, H.Kuniki and K.Yamaguchi: "Analysis of cell surface antigens using monoclonal antibodies against rat hepatocyte, particularly using HAM 1." Exp.Mol.Pathol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Kuniki, Y.Fujikura, S.Tomonaga, K.Hamano, N.Tokuda, T.Sawada, K.Kajiwara, Y.Ohba and T.Fukumoto: "Immunohistochemical localization and biological significance of the phylogenically conserved thymus-brain antigen (UB-13 antigen) in skate, rat and human." Vet.Immunol. Immunopathol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujikura,Y.et al.: "Membrane antigen expression of syngenically but heterotopically transplanted hepatocytes in rats" Cell Transplant.3. 23-31 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsuchida,M.et al.: "Analysis of cell surface antigen on glucocorticoid-treated rat thymocytes with monoclonal antibodies" Immunol.Lett.39. 209-217 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsuchida,M.et al.: "The effects of FK506 and dexamethasone on rat thymocyte differentiation" Therapeutic Immunol.1. 135-141 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsuchida,M.et al.: "Effects of irradiation,glucocorticoid,and FK506 on cell surface antigen expression by rat thymocytes: a three-color flow cytofluorometric analysis" Immunology. 83. 469-475 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Konishi,M.et al.: "Effects of FK506 on surface antigen expression by regenerating thymocytes after-sublethal irradiation in the rat" Thymus. 23. 53-66 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tokuda,M.et al.: "Changes in the distribution and intensity of alkaline phosphatase activity in rat lymph node and spleen cells after antigen stimulation" Acta Anat.151. 54-61 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fujikura,Y.et al.: "Gap Junction" Elsevier Science,Amsterdam,Netherlands,Edited by Kanno,Y., 4 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fujikura et al.: "Immunohistochemical Analysis of Rat Liver Using a Monoclonal Antibody(HAM8)Against Gap Junction." Anatomical Record. 235. 335-341 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fujikura et al.: "Immunohistochemical Characterization of Transplantable Rat Squamous Cell Carcinoma(FF-6)in Skin and Thymus" Histology and Histopathology. 8. 637-644 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fujikura et al.: "Membrane Antigen Expression of Syngenically but Heterotopically Transplanted Hepatocytes in Rats" Cell Transplantation. 3. 23-31 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fukumoto, T.: "Possible developmental interactions of hematopoletic cells and hepatocytes in fetal rat liver" Biochemical Research. 13. 385-413 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takai,K.,Tokuda,N.,Sawada, T.,Fujikura,Y.,Jojima,K., Sakatoku,J.and Fukumoto,T.: "Effects of FK506 on rat thymic epithelial cells;immunohistochemical study." Thymus. 19. 207-217 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Fujikura,Y., Ohta,H., Hirai,T. and Fukumoto,T.: "Immunochistochemical analysis of rat liver using a monoclonal antibody (HAM8) against gap junction" Anatomical Record. 235. 335-341 (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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