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The correlated analysis between the morphological and electrophysiological characteristics of the projection neurons in the reeler cerebral cortex.

Research Project

Project/Area Number 04670031
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 神経解剖学
Research InstitutionHokkaido University

Principal Investigator

INOUE Yoshiro  Hokkaido Unv.Sch. of Medicine Professor, 医学部, 教授 (20051584)

Co-Investigator(Kenkyū-buntansha) ICHIKAWA Ryoichi  Hokkaido Unv.Sch. of Medicine Instructor, 医学部, 助手 (10223091)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsReeler / Mutant / Cerebral cortex / Pyramidal cell / Dendrite / Plasticity / NMDA / reeler / mutant / cerebral cortex / pyramidal cell / dendrite / リーラーマウス / 投射ニューロン / 大脳皮質運動領 / 皮質脊髄路ニューロン / 皮質視床ニューロン / 皮質交連ニューロン
Research Abstract

The plasticity of the signal transduction system intrinsically present in the projection neurons was investigated using the reeler cerebral and cerebellar cortex by means of the morphomeiric study, electrophysiological analysis by the slice-method and in situ hybridization method for NMDA receptor channel. The results obtained were as follows : (1)The corticospinal, corticothalamic and corticocommissural neurons of the reeler and normal mice were retrogradely labeled by biocytin and the entire shape of the labeled neurons were input into the personal computer system through the degitizer and the morphometric calculation was carried out. The morphological characteristics of the projection neurons in the normal and reeler was possibly determined by their own projection sites, whereas the extension pattern of the apical dendrites was influenced in their location within the cerebral columna and input factors from other neurons. (2)The electrophysiological equipment was prepared for the brain-slice methos. The techniques for slicing brain, pulling the electrical micropipettes and labelling neurons in the slice were established using the normal mice. (3)By in situ hybridization study for the NMDA receptor channel the Purkinje cell in the reeler cerebellum revealed anomalous expression with special reference to the topological distribution. This means the NMDA recetor channel possibly have an intrinsic variability influenced by the synaptic environment even in the cerebral cortex. (4)The reelet neurons in the cerebrum and cerebellum were highly useful for the investigation of the intrinsic factors for expressing transduction system within the nerve cells and plasticity and variability of the neuronal circuits.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Inoue,K.: "“Postnatal development of the corticotectal projection from the vixual corlex of the mouse"" Okajimas Folia Anat.Jpn.68. 319-332 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Terashima,T.: "“Dendritic arbolization of large pyramidal neurons in the motor cortex of normal and reeler mutant mouse."" Okajimas Folia Anat.Jpn.68. 351-364 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Watanabe,M.: "“Developmental changes in distribution of NMDA receptor channel subunit mRNAs"" Neuroreport. 3. 1138-1140 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Terashima,T.: "“Distribution of guanine nucleotide-binding protein in the brain of reeler mutant mouse"" Brain Res.601. 136-142 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue,Y.: "“Abnormal synaptic architecture in the cerebellar cortex of a new dystonic mutant mouse,Wriggle Mouse Sagami."" Neuroscience Res.16. 39-48 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Watanabe,M.: "“Distinct distributions of five N-methyl-D-aspartate receptor channel subunit mRNAs in the forebrain."" J.Comp.Neurol.338. 377-390 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue, K.: "Postnatal development of the corticotecial projection from the vixual cortex of the mouse"" Okajimas Folia Anat.Jpn.68. 319-332 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Terashima, T.: "Dendritic arbolization of large pyramidal neurons in the motor cortex of normal and reeler mutant mouse." Okajimas Folia Anat.Jpn.68. 351-364 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Watanabe, M.: "Developmental changes in distribution of NMDA receptor channel subunit mRNAs." Neuroreport. 3. 1138-1140 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ikeda, K.: "Cloning and expression of the epsilon 4 subunit mRNA of the NMDA receptor channel." FEBS lett. 313. 34-38 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Terashima, T.: "Distribution of guanine nucleotide-binding protein in the brain of reeler mutant mouse." Brain Res.601. 136-142 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue, Y.: "Abnormal synaptic architecture in the cerebellar cortex of a new dystonic mutant mouse, Wriggle Mouse Sagami." Neuroscience Res.16. 39-48 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tsutsui, K.: "Molecular cloning of partial cDNAs for rat DNA topoisomeraseII isoforms and their differential expression in brain development." J Biological Chem.268. 19076-19083 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Watanabe, M.: "Distinct distributions of five N-methyl-D-aspartate receptor channel subunit mRNAs in the forebrain." J.Comp.Neurol.338. 377-390 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Araki, K.: "Selective expression of the glutamate receptor channel delta 2 subunit in cerebellar Purkinje cells." Biochemical and Biophysical Research Communication. 197(3). 1267-1276 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Watanabe, M.: "Differential distribution of the NMDA receptor channel subunit mRNAs in the mouse retina." Brain Research. 634. 328-332 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 池中一裕: "『神経疾患の動物モデル--ミエリンプロテオリピド蛋白質遺伝子過剰発現によるミエリン形成不全および脱髄マウスの作製--』" 最新医学. 48. 1560-1566 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Araki K: "“Selective expression of the glutamate receptor channel δ2 subunit in cerebellar Purkinje cells."" Biochemical and Biophysical Research Communication. 197. 1267-1276 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 井上芳郎: "『錐体路の発生学的研究より』" 脳神経外科速報. 3. 265-269 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Inoue,Y: "“Abnormal synaptic architecture in the cerebellar cortex of a new dystonic mutant mouse,Wriggle Mouse Sagami."" Neuroscience Res.16. 39-48 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Watanabe M: "“Distinct distributions of five N-methyl-D-aspartate receptor channel subunit mRNAs in the forebrain."" J.Comp.Neurol.338. 377-390 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Watanabe M: "“Differential distribution of the NMDA receptor channel subunit mRNAs in the mouse retina."" Brain Research. 634. 328-332 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Watanabe,M.: "Developmental changes in distribution of NMDA receptor channel subunit mRNAs" Neuroreport. 3. 1138-1140 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Inoue,K.: "Postnatal development of the corticotectal projection from the visual cortex of the mouse"" Okajimas Folia Anat.Jpn.68. 319-332 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Terashima,T.: "Distribution of guanine nucleotide-binding protein in the brain of reeler mutant mouse"" Brain Res.601. 136-142 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Terashima,T.: "Dendritic arbolization of large pyramidal neurons in the motor cortex of normal and reeler mutant mouse."" Okajimas Folia Anat.Jpn.68. 351-364 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Inoue,Y.: "Abnormal synaptic architecture in the cerebellar cortex of a new dystonic mutant mouse,Wriggle Mouse Sagami."" Neuroscience Res.(1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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