| Project/Area Number |
04670136
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
ISHII Tetsuro Inst.Basic Med.Sci., Associ Prof., 基礎医学系, 助教授 (20111370)
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| Co-Investigator(Kenkyū-buntansha) |
BANNAI Shiro Inst.Basic Med.Sci., Prof., 基礎医学系, 教授 (70019579)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Cystine / Stress protein / Amino acid transport / cDNA / Macrophage / グルタミン酸 |
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| Research Abstract |
Stress agents induces cystine transport activity in mouse macrophages cultured in vitro. The induction of cystine entry into the cells enhances synthesis of glutathione, an anti-oxidative cellular substance. We tried to clone the carrier of cystine by the differential screening to select stress-induced clones. We constructed a directional cDNA library from 1.5-3 kb fraction of mouse macrophage mRNA.We selected total 800 clones from about 8x10^4 phage plaques. We could not detect any expression of the cystine transport activity in Xenopus laevis oocytes after microinjection of cRNAs synthesized from those cDNAs.
Although we could not isolate a cNDA clone encodnig cystine carrier, we have succeeded in the cloning of a stress-induced 23 kDa protein named MSP23. This protein belongs to a new type of antioxidative proteins. Additionally we found that two other known proteins were induced upon exposure to oxidative stress. We also obtained a few cDNA clones that encode novel stress-induced proteins.
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