| Project/Area Number |
04670158
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IZUMI Takashi TOKYO UNIV., DEPT.OF MEDICINE, ASSISTANT, 医学部(医), 助手 (70232361)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Zen-ichiro TOKYO UNIV., DEPT.OF MEDICINE, ASSISTANT, 保健センター, 助手 (70238814)
SHIMIZIU Takao TOKYO UNIV., DEPT.OF MEDICINE, PROFESSOR, 医学部(医), 教授 (80127092)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | BIOACTIVE LIPIDS / LEUKOTRIENE / PLATELET-ACTIVATING FACTOR / SYNTHETIC ENZYME / RECEPTOR / SIGNAL TRANSDUCTION / INACTIVATING ENZYME |
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| Research Abstract |
1. STUDY FOR LEUKOTRIENE BIOSYNTHESIS AND METABOLISM
A monoclonal antibody to LTC_4 synthase was developed, and using this antibody enzyme purification is now going on.
A mutagenesis study of LTA_4 hydrolase revealed that the active center for LTB_4 producing activity is different from that for aminopeptidase activity.
We showed that LTB_4 is inactivated by LTB_4-12-hydroxydehydrogenase in porcine kidney, and this enzyme was purified to apparent homogeneity.
2. STUDY FOR FUNCTION OF LEUKOTRIENE
Studies for cDNA cloning of leukotriene receptors are going on using frog oocyte expression system or mammalian cell expression system.
3. STUDY FOR PAR RECEPTOR
The presence of functionally active PAF receptor in rat brain have been indicated.
In a human eosinophilic leukemia cell line (EoL-1 cells), PAF receptor were induced during differentiation by sodium butyrate.
Studies on human PAF receptor gene revealed that two different promoter exist to produce two types of mRNA, namely leukocyte type and heart type.
In CHO cells transfected with PAF receptor, PAF activated MAP kinase and MAP kinase kinase.
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