| Project/Area Number |
04670169
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Tokai University School of Medicine |
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Principal Investigator |
YOSHIMURA Shinichi Assistant Professor Department of Molecular Life Science, 医学部, 講師 (30230808)
|
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| Co-Investigator(Kenkyū-buntansha) |
MORIUCHI Tetsuya Associate Professor Department of Molecular Life Science, 医学部, 助教授 (20174394)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | glutathione peroxidase / gene / chromosome mapping / human / plasma type / selenium / 腎臓 / セレニウム |
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| Research Abstract |
A genomic clone encoding the human plasma glutathione peroxidase (PGPx), amajor enzyme reducing lipid hydroperoxide and hydrogen peroxide in plasma, was isolated and 5618 nucleotides were determined. The nucleotide sequence data revealed that the PGPx gene is composed of five exons spanning approximately 10 kilobases. Primer extension experiments mapped the transcription start point at 298 nucleotides upstream from the predicted translation initiation codon. Seventeen nucleotides upstream from the polyadenylation site of the gene, an uncanonical polyadenylation signal, AGTAAA, was found. The human PGPx gene was localized on chromosome 5 band q32 by fluorescence in situ hybridization.
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