| Project/Area Number |
04670184
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Saitama Medical School |
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Principal Investigator |
SUGAWARA Isamu Saitama Medical School, Medicine Associate Professor, 医学部, 助教授 (10196701)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Diabetes mellitus / beta cell / specific antigen / beta細胞 / モノクローナル抗体 / 分子病理診断 |
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| Research Abstract |
5D-4 murine morine monoclonil antibody reacts with islets of Langerhans. Strictly speaking, MAb 5D04 reacts with 3okDa, 5ukDa antigens from islets of Langerhans. The antigens were reduced in type 1 and II diabetes mellitus, but not in cancer-associated diabetes mellitus ( secondary diabetes ). Amino aad sequence of 3okDa antigen was determined parfially by a peptide synthezer. The 3okDa antigen was obtained from immunoelectrophres of Aspc-1-devived solubilized proteins and subsequent HPLC.
mRNA expression of 3okDa protein was also confirmed by RT-PCR using Aspc-1 cells ( pancreatic cancer cell line ).
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