• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Histiocytic proliferative lesions of soft tissues : True nature and cell kinetics.

Research Project

Project/Area Number 04670193
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionFukuoka University

Principal Investigator

IWASAKI Hiroshi  Fukuoka University, Department of Pathology, Professor, 医学部, 教授 (90101170)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsHistiocyte / Fibroblast / Malignant fibrous histiocytoma / Sarcoma / Soft tissue / Connective tissue / Mesenchymal tissue / Cell proliferation / 軟部腫瘍 / 線維性組織球腫 / 染色体 / 免疫組織化学
Research Abstract

Histiocytic proliferative lesions of soft tissues show a wide spectrum of histologic features, ranging from reactive inflammatory lesions to fibrohistiocytic tumors. To elucidate the origin and characteristics of the lesions, we analyzed surgical materials of malignant fibrous histiocytoma (MFH) by single and double immunostainings, using histocytic, mesenchymal and proliferating cell markers. As controls, benign fibrous histiocytoma (dermatofibroma), and giant cell tumor of tendon sheath were studied by the same procedure.
All three types of MFH cells (spindle cells, polygonal cells and bizarre giant cells) stained positive for mesenchymal antigens (FU3 and vimentin) but did not stain for macrophage/histiocyte markers (HAM 56 and CD68). Therefore, the MFH cells may not represent true histiocytes, although they may be mesenchymalderived cells behaving as "facultative histiocytes" with superficial resemblance to actual histiocytes. Normal histiocytes in the stroma showed varying degrees … More of positivity for macrophage/histiocyte antigens. Proliferating cell nuclear antigen (PCNA) and MIB-1 were expressed by all the three cell types of MFH cells. Expression of p53 protein was found in 50% of MFH, but its positivity varied greatly from tumor to tumor.
In addition, 17 MFH cell lines were analyzed by using light- and electron-microscopy, immunocytochemistry, and functional tests for Fc receptors and immunophagocytosis. Cultured MFH cells exhibited a storiform/pleomorphic pattern, corresponding to the histologic features of the primary tumors. The cells in each line displayd histiocytic functional markers such as lysosomal enzymes, Fc receptors, and immunophagocytosis. However, MFH cells expressed a mesenchymal antigen (FU3) that is distributed among perivascular cells and fibroblasts, whereas MFH cells demonstrated no positive reactions for CD15 (Leu M1) and CD14 (Leu M3) that recognize the cells of the monocyte-macrophage lineage. These findings suggest that MFH is not a tumor of true histiocytes but of "facultative histiocytes" showing both mesenchymal differentiation and histiocytic functional makers in vitro. Chromosomal analysis demonstrated complex abnormal karyotypes varying greatly among the MFH tumors examined.
By DNA flow cytometry, 76% of MFH showed aneuploidy and these tumors exhibited poor prognosis (10-year survival, 55.3%) when compared with diploid tumors (10-year suvival, 92.3%). Less

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Iwasaki,H.,Isayama,T.: "Malignant fibrous histiocytoma:A tumor of facultative histiocytes showing mesenchymal differentiation in cultured cell lines." Cancer. 69. 437-447 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Iwasaki,H.,Yoshitake,K.: "Malignant fibrous histiocytoma:proliferative compartment and heterogeneity of“histiocytic"cells." Am J Surg Pathol. 16. 735-745 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ohjimi,Y.,Iwasaki,H.: "Myxoid liposarcoma with t(12;16)(q13;p11).Possible usefulness of chromosome analysis in a poorly differentiated sarcoma." Pathol Res Pract. 188. 736-741 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ohjimi,Y.,Iwasaki,H.: "A case of lipoblastoma with t(3;8)(q12;q11.2)" Cancer Genet Cytogenet. 62. 103-105 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ohjimi,Y.,Iwasaki,H.: "Chromosome abnormalities in liposarcomas." Cancer Genet Cytogenet. 64. 111-117 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Emoto,M.,Iwasaki,H.: "Two cell lines established from mixed mullerian tumors of the uterus:morphologic,immunocytochemical,and cytogenetic analyses." Cancer. 69. 1759-1768 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Emoto,M.,Iwasaki,H.: "Characteristics of cloned cells of mixed mullerian tumors of the human uterus." Cancer. 71. 3067-3075 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ohjimi,Y.,Iwasaki,H.: "Deletion(X)(p11):Another case of renal adenocarcinoma with involvement of Xp11." Cancer Genet.Cytogenet.70. 77-78 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Watanabe,R.,Jhozaki,H.,Iwasaki,H.,Kikuchi,M.: "A new tumor-associated antigen defined by a monoclonal antibody directed to gastric adenocarcinoma." Cancer. 71. 2440-2447 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Li,D-F.,Iwasaki,H.: "Dermatofibroma:Superficial fibrous proliferation with reactive histiocytes.A multiple immunostaining analysis." Cancer. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 岩崎宏: "悪性線維性組織球腫“病理組織診断における電子顕微鏡の有用性"" 病理と臨床. 10(臨時増刊). 420-421 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 岩崎 宏(分担執筆) (遠城寺宗知編): "現代病理学体系20軟部腫瘍" 中山書店,東京, 48 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 岩崎 宏(分担執筆) (藍沢,清水,里美編): "取扱い規約に沿った腫瘍鑑別診断アトラス 軟部" 文光堂,東京, 9 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 岩崎 宏(分担執筆): "取扱い規約に沿った腫瘍鑑別診断アトラス 腎臓(牛込,町並編)" 文光堂,東京, 7 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Iwasaki, H., Isayama, T., Ohjimi, Y., Kikuchi, M., Yoh, S., Shinohara, N., Yoshitake, K., Ishiguro, M., Kamada, N., and Enjoji, M.: "Malignant fibrous histiocytoma : a tumor of facultative histiocytes showing mesenchymal differetiation in cultured cell lines." Cancer. 69. 437-447 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Iwasaki, H., Yoshitake, K., Ohjimi, Y., Kikuchi, M., Isayama, T., Yoh, S., Shinohara, N., and Enjoji, M.: "Malignant fibrous histiocytoma : proliferative compartment and heterogeneity of "histiocytic" cells." Am.J.Surg.Pathol. 16. 735-745 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ohjimi, Y., Iwasaki, H., Ishiguro, M., Ohgami, A., Yoshitake, K., Fijita, C., Kikuchi, M., Shinohara, N., and Kaneko, Y. : Myxoid liposarcoma with t(12 ; 16)(q13 ; p11): "possible usefulness of chromosome analysis in a poorly differentiated sarcoma." Pathol.Res.Pract.188. 736-741 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ohjimi, Y., Iwasaki, H., Kaneko, Y., Ishiguro, M., Ohgami, A., and KIkuchi, M.: "A case of lipoblastoma with t(3 ; 8)(q12 ; 11.2)." Cancer Genet.Cytogenet.62. 103-105 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ohjimi, Y., Iwasaki, H., Kaneko, Y., Ishiguro, M., Ohgami, A., Fujita, C., Shinohara, N., Yoshitake, K., and Kikuchi, M.: "Chromosome abnormalities in liposarcomas." Cancer Ganet.Cytogenet.64. 111-117 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Emoto, M., Iwasaki, H., Kikuchi, M., Ishiguro, M., Kubota, T., Izumi, H., Shirakawa, K., and Kaneko, Y.: "Two cell lines established from mixed mullerian tumors of the uterus : Morphologic, immunocytochemical, and cytogenetic analyzes." Cancer. 69. 1759-1768 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Emoto, M., Iwasaki, H., Kikuchi, M., and Shirakawa, K.: "Characteristics of cloned cells of mixed mullerian tumor of the human uterus : Carcinoma cells showing myogenic differentiation in vitro." Cancer. 71. 3065-3075 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Ohjimi, Y., Iwasaki, H., Ishiguro, M., Hara, H., Ohgami, A., Kikuchi, M., and Kaneko, Y. : Delection (X)(p11): "Another case of renal adnocarcinoma with involvement of Xp11." Cancer Genet.Cytogenet.70. 77-78 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Watanabe, R., Jhozaki, H., Iwasaki, M., and Ikeda, S.: "A new tumor-associated antigen defined by a monoclonal antibody directed to gastric adenocarcinoma." Cancer. 71. 2440-2447 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Li, D-F., Iwasaki, H., Kikuchi, M., Ichii, M., Ogata, K.: "Dermatofibroma : Superficial fibrous proliferation with reactive histiocytes--A multiple immunostaining analysis." Cancer. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] LiD,Iwasaki H,et al: "Dermatotibroma:Superficial fibrous proliferation with reactive histiocytes.A multiple immunostaining analysis." Cancer. 印刷中. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Emoto M,Iwasaki H,et al: "Characteristics of cloned cells of mixed mullerian tumors of the human uterus." Cancer. 71. 3067-3075 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohjimi Y,Iwasaki H,et al: "Chromosome abnormalities in liposarcomas." Cancer Genet Cytogenet. 64. 111-117 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Iwasaki H,Isayama T,Ohjimi Y,Kikuchi M: "Malignant fibrous histiocytoma: A tumor of facultative histiocytes showing mesenchymal differentiation in cultured cell lines." Cancer. 69. 437-447 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Iwasaki H et al: "Malignant fibrous histiocytoma:proliferative compartment and heterogeneity of"histiocytic"cells." Am J Surg Pathol. 16. 735-745 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Emoto M,Iwasaki H,Ishiguro M,Kubota T: "Two cell lines established from mixed mullerian tumors of the uterus:morphologic,immunocytochemical,and cytogenetic analyses." Cancer. 69. 1759-1768 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohjimi Y,Iwasaki H,Ishiguro M,Ohgami A: "Myxoid liposacoma with t(12;16)(q13;p11).Possible usefulness of chromosome analysis in a poorly differentiated sarcoma." Pathol Res Pract. 188. 736-741 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohjimi Y,Iwasaki H,Ishiguro M,Kikuchi M: "A case of lipoblastoma with t(3;8)(q12;q11.2)" Cancer Genet Cytogenet. 62. 103-105 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 岩崎,宏: "悪性線維性組織球腫"病理組織診断における電子顕微鏡の有用性"" 病理と臨床. 10・(臨時増刊). 420-421 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] 岩崎,宏: "取り扱い規約に沿った腫瘍鑑別診断アトラス:腎臓" 文光堂, 7 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 岩崎,宏: "取り扱い規約に沿った腫瘍鑑別診断アトラス:軟部" 文光堂, 7 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 岩崎,宏: "現代病理学体系20軟部腫瘍(遠城寺宗知編)" 中山書店, 48 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Iwasaki H,Isayama T,Ohjimi Y,Kikuchi M: "Malignant fibrous histiocytoma:A tumor of facultative histiocytes showing mesenchymal differentiation in cultured cell lines." Cancer. 69. 437-447 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Iwasaki H et al: "Malignant fibrous histiocytoma:proliferative compartment and heterogeneity of “histiocytic"cells." Am J Surg Pathol. 16. 735-745 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Emoto M,Iwasaki H,Ishiguro M,Kubota T: "Two cell lines established from mixed mullerian tumors of the uterus:morphologic,immunocytochemical,and cytogenetic analyses." Cancer. 69. 1759-1768 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ohjimi Y,Iwasaki H,Ishiguro M,Ohgami A: "Myxoid liposarcoma with t(12;16)(q13;p11).Possible usefulness of chromosome analysis in a poorly differentiated sarcoma." Pathol Res Pract. 188. 736-741 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ohjimi Y,Iwasaki H,Ishiguro M,Kikuchi M: "A case of lipoblastoma with t(3;8)(q12;q11.2)" Cancer Genet Cytogenet. 62. 103-105 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 岩崎 宏: "悪性線維性組織球腫“病理組織診断における電子顕微鏡の有用性"" 病理と臨床(臨時増刊). 10. 420-421 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 岩崎 宏: "現代病理学体系20軟部腫瘍(遠城寺宗知編)" 中山書店, 48 (1992)

    • Related Report
      1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi