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Effect of forced N-myc gene expression on src gene expression in neuroblastoma cells

Research Project

Project/Area Number 04670197
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionChiba University

Principal Investigator

SIMIZU Bunsiti  Chiba Univ., School of medicine, Professor, 医学部, 教授 (10072894)

Co-Investigator(Kenkyū-buntansha) SHIRASAWA Hiroshi  Chiba Univ., School of medicine, Instructor, 医学部, 助手 (00216194)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsNeuroblastoma / N-myc / src
Research Abstract

NB69N which is a clonal subline of neuroblastoma cell line NB69, has a neuroblastic characteristic and an ability to differentiate by retinoic acid. S1 nuclease protection assay showed that NB69N expressed not only nonneuronal c-src but neuronal c-srcN1 and c-srcN2 mRNA.Because NB69N cell has a single copy of N-myc gene and does not express N-myc mRNA, we introduced N-myc expression vector into the NB69N cells by an electroporation method. We cloned 7 N-myc transfectants (NB69N41 - 47) and one control transfectant NB69NR.In 7 N-myc transfectants, the exogenous N-myc DNA was integrated in genomic DNA, and N-myc mRNA and protein products from the introduced N-myc were expressed. NB69N cells had no advantage of growth activity in a normal culture medium, but acquired a resistance to serum starvation. Furthermore, NB69N47 cells were able to form 6 times colonies to compare with NB69NR in soft agar. No difference of c-src mRNA splicing and expression levels was observed between NB69N47 and NB69NR by S1 nuclease protection assays. The forced expression of N-myc gene possibly conferred a malignant potential to the neuroblastoma cells independent of the c-src mRNA expression.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report

Research Products

(15 results)

All Other

All Publications

  • [Publications] T.Matsunaga et al.: "Paratesticular neuroblastoma with N-myc activation" J.Pediatric Surgery. 28. 1612-1614 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsunaga et al.: "Expression of alternatively spliced src messenger RNAs related to neuronal differentiation in human neuroblastomas" Cancer Research. 53. 3179-3185 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] H.Shirasawa et.al.: "Transcription modulatory activity of full-length E6 and E6^〓I proteins of human papillomavirus type16" Virology. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] K.Shimizu et al.: "Alternative splicing of human papillomavirus type16 E6 mRNA in mouse and monkey cell lines" International Journal of Oncology. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tadashi Matsunaga et al.: "Expression of Alternatively spliced src messenger RNAs related to neuronal differentiation in human neuroblastomas." Cancer Research. 53. 3179-3185 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Tadashi Matsunaga et al.: "Paratesticular neuroblastoma with N-myc activation" J.Ped.Surg.28. 1612-1614 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kumiko Shimizu et al.: "Alternative splicing of human papillomavirus type-16 E6-mRNA in mouse and monkey cell lines." Int.J.Oncology. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hiroshi Shirasawa et al.: "Transcription-modulatory activity of full-length E6 and E6**1 proteins of human papillomavirus type 16." Virology. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Matsunaga et al.: "Paratesticular neuroblastoma with N-myc activation" J.Pediatric Surgery. 28. 1612-1614 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Matsunaga et al.: "Expression of alternatively spliced src mRNAs in human neuroblastomas related to neuronal diff." Cancer Research. 53. 3179-3185 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Shirasawa et al.: "Transcription-modulatory activity of full-length E6 and E6 I protains of human papillomavirus type 16" Virology. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Shimizu et al.: "Alternative splicing of human papillomavirus type 16 E6 mRNA in mouse and monkey cell lines" International Journal of Oncology. (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] H.Shirasawa: "Typing of human papillomavirus" Japanese archives of sexually Transmitted disease. 2. 55-60 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.Sekiya et al.: "Expression of a human multidwg resistance geno in human ovarian carcinoma cell lines" Arch Gynecol.Obstet.251. 79-86 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Furuya et al.: "Loss of androgen dependency with preservation of functional androgen receptors in androgen-dependent mouse Tumor(Shionogi carcinoma 115)" The Journal of Steroid Biochemistry and Molecular Biology. 42. 569-574 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-03-31   Modified: 2016-04-21  

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