| Project/Area Number |
04670206
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Osaka University |
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Principal Investigator |
NOMURA Shintarou School of Medicne, Osaka University*, 医学部, 助教授 (80159087)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Fructue Healing / BMP-4 / Geine Expression / Bone matrix / Osteopontin / Callus / Development / Calcification / BMP / in situハイブリダイゼーション / オステオネクチン / 骨芽細胞 |
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| Research Abstract |
The protein composition and the morphology of the callus which appear in the process of fracture healing is different from matured bone bat resembles to embryomic bone. Osteoblastic cells which are to form callus expressing Osteopontin and Osteonectin mRNA, of which level is low in the matured bone. We demonstrated that by in situ hybridization and Northern hybridization. BMP-4 (Bone Morhpogenetic Protein 4) can induce a ectopicbone when injected under the muscles of mice. The morphogenic structure of the ectopic bone is resemble to the callus. This observation strongly suggest that BMP-4 express in embryonic bone and callus. This was demonstrated by in situ hybridization. Furthermore we demonstrated that in phthological calcification, also the expression of Osteopontin mRNA is essential (i. e. artheloscreliosis, breast cancer. urinary stone formation). This result indicate that there is a general pathway of calcification not only bone and teeth but also pathological calcification. And the expression of Osteopontin is always involved in the process.
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