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Influence of cell-proliferating stimuli on carcinogen-induced chromosome aberrations and sister chromitid exchanges

Research Project

Project/Area Number 04670212
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionEhime university

Principal Investigator

UEDA Norifumi  Ehime university School of Medicine, Professor, 医学部, 教授 (30030886)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsChromosome aberrations / Sister chromatid exchanges / Carcinogen / Cell growth factor / 変異原・癌原物質 / 増殖因子 / 変異原物質 / 化学発癌剤 / 染色体異常 / 標的細胞
Research Abstract

Acute chromosome-damaging and chromatid-rearranging capacities of various mutagens and carcinogenes such as 7,12-dimethylbenz(a)anthracene(DMBA), and N-nitroso-N-methylurea(NMU) were studied with rat bone marrow cells, liver cells and fibroblasts in vivo and in vitro. The frequency of chromosome aberrations(CA) and sister chromatid exchange(SCE) after administration of above-mentioned carcinogens was tested under various stimuli. The incidence of DMBA-and NMU-induced CA in the anemic rats was 45% and 56%, and in the polycythemic rats, it was 17% and 24%(normal rats 28-33%). However, the incidence of CA in polycythemic rats inoculated with 6 Units of erythropietin at the time of DMBA injection was 41% and 47%. In the other words, the percentage of metaphase cells with CA induced by DMBA was enhanced by anemia andsuppressed by polycythemia. The low incidence of CA in polycythemic rats was re-versed by 6 units of sheep erythropoietin injected at the time of DMBA and NMU treatment. CA were distributed nonrandomly in chromosomes 1 and 2. One region, the 40% region from the centromere(1q31), of the long arm of chromosome 1, and three regions, 30, 55 and 80% from the centromere(2q21, 2q25, 2q41), of chromosome 2 were extremely susceptible to DMBA and NMU in the normal hematopietic state. Also, the enhancing effect of erythropoietin on DMBA- and NMU- induced CA was considered specific with respect to chromosomal regions. The frequency of SCE was increased under the condition of anemia and that of SCE was decreased under the condition of polycythemia. In liver cell obtained hepatectomized rats, the frequency of SCE induced by DMBA was enhanced with addition of the liver cell growth factor in vivo(from 13.5% to 28.3%). In fibroblasts, the frequency of SCE induced by NMU was enhanced in the ratio of 1.5 times with addition of thefibroblastic growth factor in cultured medium in vitro.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 植田規史・村尾真一: "増殖刺激下の標的細胞における変異原癌原物質の急性染色体傷害作用の増強作用" 病態と生理. 12. 734-738 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 木藤克己.木花敏雅.明比俊.松元隆.村尾真一.植田規史: "DMBA及びNMU発癌ラットにおける癌抑制遺伝子p53の点突然変異の検討" 第52回日本癌学会総会記事. 52. 243 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 木藤克己.木花敏雅.植田規史.杉田敦郎.村尾真一.福西亮: "ヒト及び化学発癌ラット乳癌におけるp53遺伝子の点突然変異の検討" 日本病理学会会誌. 82. 251 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 木藤克己.木花敏雅.植田規史.青野幸治.宮内勝敏.木村茂: "神経芽細胞におけるc-ki-ras,N-ras遺伝子の検討" 小児がん. 29. 295-297 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 武井由美.木藤克己.杉田敦郎.植田規史: "ヒト乳癌組織におけるp53蛋白の発現" Ehime.Med.J. 11. 9-11 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 木藤克己.木花敏雅.清水英範.植田規史.木村茂.福西亮: "ヒト乳癌における癌抑制遺伝子の点突然変異の検討" 第51回日本癌学会総会記事. 51. 131 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 植田規史: "標準病理学.染色体.遺伝子及び発生の異常" 医学書院(印刷中), (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Norifumi Ueda and Shin-ichi Murao: "Influence of proliferating stimuli by cell growth factors on carcinogen-induced chromosome aberrations and sister chromatid exchanges in rat bone marrow cells, fibroblasts and liver cells." Medicina Philosophica. 12. 734-738 (1933)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Katsumi Kito, Toshimasa Kihana, Shun Akehi, Takashi Matsumoto, Shin-ichi Murao, Shigeru Kimura and Norifumi Ueda: "p53 tumor suppressor gene mutations in rat mammary tumors induced by DMBA." Proc.Jap Cancer Assoc. 52. 243 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Katsumi Kito, Toshimasa Kihana, Norifumi Ueda, Atsuro Sugita, Shin-ichi Murao & Ryo Fukunishi: "p53 tumor suppressor gene mutations in human and chemical-induced mammary cancer." Trans Soc Pathol Jap. 82. 251 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Katsumi Kito, Toshimasa Kihana, Norifumi Ueda, Kouji Aono, Katsutoshi Miyauchi, Shigeru Kimura and Ryo Fukunishi: "point mutations of p53, K-ras and N-ras gene in neuroblastoma." Pediatr.cancer. 29. 295-297 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yumi Takei, Katsumi Kito, Atsuro Sugita and Norifumi Ueda: "p53 expression in breast cancer." Ehime Med J. 11-9-11 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Katsumi Kito, Toshimasa Kihana, Hidenori Shimizu, Norifumi Ueda, Shigeru Kimura and Ryo Fukunishi: "Tumor suppressor gene mutations in human breast cancer." Proc Jap Cancer Assoc. 51. 131 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Norifumi Ueda: "Chromosomal, genetical and developmental anomalies." Standard Pathology, (ed, Machinami R.). (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 植田規史 村尾真一: "増殖刺激下の標的細胞における変異原・癌原物質の急性染色体傷害作用の増強作用" 病態と生理. 12. 734-738 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 木藤克己.木花敏雅.明比〓,松元隆.村尾真一.植田規史: "DMBA及びNMU発癌ラットにおける癌抑制遺伝子p53の点突然変異の検討" 第52回日本癌学会総会記事. 52. 243 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 木藤克己 木花敏雅 植田規史 松田敦郎 村尾真一 福西亮: "ヒト及び化学発癌ラットにおけるp53遺伝子の点突然変異" 日本病理学会会誌. 82. 251 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 植田規史, 町並陸生編: "標準病理学.染色体.遺伝子及び発生の異常" 医学書院(印刷中), (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 木藤 克己: "神経芽細胞における癌抑制遺伝子p53及び癌関連遺伝子K-ras,N-rasの点突然変異の検討" 日本病理学会会誌. 81. 240 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 木藤 克己: "乳癌における癌抑制遺伝子p53の点突然変異の検討" 日本癌学会総会記事. 51. 131 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 杉田 敦郎: "乳癌における抗血管増殖因子の作用" 日本癌学会総会記事. 51. 306 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 武井 由美: "ヒト乳癌におけるp53蛋白の発現" 愛媛医学. 11. 69-71 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 木藤 克己: "神経芽細胞腫におけるp53遺伝子及びK-ras N-rasの点突然変異の検討" 小児がん. 29. 295-297 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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