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Accumulation of alphaB-crystallin in glioma cells and the relation to oncogene expression.

Research Project

Project/Area Number 04670213
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

IWAKI Toru  Kyushu University, Faculty of Medicine, Lecturer, 医学部, 講師 (40221098)

Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsalphaB-ctystallin / heat shock protein / brain tumor / glioma / regulation of expression / oncogene / alphaB-クリスタリン
Research Abstract

We have identified a major component of Rosenthal fibers as alphaB-crystallin, a major lens protein. The amount of alphaB-crystallin normally present in the brain is small, but it accumulates in reactive and neoplastic glial cells and in some neurons in a variety of pathologic conditions. Thus, the accumulation of alphaB-crystallin appears to be a part of a reactive process and is required for the formation of Rosenthal fibers. To understand the significance of the accumulation of alphaB-crystallin in Rosenthal fibers within astrocytes, the expression and metabolism of alphaB-crystallin in glioma cell lines were examined under the condition of heat and oxidative stress. alphaB-crystallin mRNA was increased after both stresses and alphaB-crystallin protein moved from a detergent-soluble to a detergent-insoluble form. Next we investigated the phenotypic effects of selectively altering the levels of alphaB-crystallin in cultured glial cells by genetic manipulation. Rat C6 glioma cells and human U-373MG glioma cells were transfected with a rat alphaB-crystallin sense cDNA or an antisense cDNA to alter cellular levels of alphaB-crystallin. The anti-sense strategy resulted in decreased alphaB-crystallin levels, as revealed by Western blot and immunocytochemical analyzes. The reduced alphaB-crystallin expression was accompanied by alterations in cellular phenotype : (1)a reduction of cell size and/or a slender cell morphology ; (2)a disorganized microfilament network ; and (3)a reduction of cell adhensiveness. Like HSP27, the presence of additional alphaB-crystallin protein confers a thermoresistant phenotype to stable tranfectants. Thus, alphaB-crystallin in glioma cells plays a role in their thermal resistance and may contribute to the stability of cytoskeletal organization.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Iwaki,T.: "αB-crystallin and 27-kd heat shock protein are regulated by stress conditions in the central nervous system and accumulate in Rosenthal fibers." Am.J.Pathol.143. 487-495 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yokoyama,N.: "Small heat-shock protein is expressed in menigiomas and in granulofilamentous inclusion bodies." Acta Neuropathol.85. 248-255 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Iwaki, A.Iwaki, J.Tateishi, Y.Sakaki, J.E.Goldman: "alphaB-crystallin and 27-kd heat shock protein are regulated by stress conditions in the central nervous system and accumulate in Rosenthal fibers." Am.J.Pathol.143(2). 487-495 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] N.Yokoyama, T.Iwaki, J.E.Goleman, J.Tateishi, M.Fukui: "Small heat-shock protein is expressed in meningiomas and in granulofilamentous inclusion bodies." Acta Neuropathol.85(3). 248-255 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Iwaki,T.: "alphabeta-crystallin and 27-kd heat shock protein are regulated by stress conditions in the central nervous systemand accumulate in Rosenthal fibers." Am.J.Pathol.143. 487-495 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yokoyama,N.: "Small heat-shock protein is expresseds in menigiomas and in granulofilamentous inclusion bodies." Acta Neuropathol.85. 248-255 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Iwaki,T.: "αB-crystallin and 27 kilodalton heat shock protein are regulated by stress conditions in the central nervous system." Am.J.Pathol.

    • Related Report
      1992 Annual Research Report
  • [Publications] Yokoyama,N.: "Small heat-shock protein is expressed in meningiomas and in granulo-filamentous inclusion bodies." Acta Neuropathol.

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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