| Project/Area Number |
04670223
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学）
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| Research Institution | Akita University |
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Principal Investigator |
YOSHIMURA Kentaro Akita University, Professor, 医学部, 教授 (90053058)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Hiroko Akita University, Res. Associate, 医学部, 助手 (30235626)
ISHIDA Kazuto Akita University, Res. Associate, 医学部, 助手 (60006731)
ABE Tatsuya Akita University, Assoc. Professor, 医学部, 助教授 (80128363)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Angiostrongylus cantonensis / Mesocestoides corti / eosinophils / cerebrospinal fluid / nude mice / blood-brain barrier / eosinophil chemotactic factor / 好酸球遊走因子 |
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| Research Abstract |
Results obtained are summatrized as follows : 1. Eosinophil accumulation in nonpermissive host (mice) : Cerebrospinal fluid (CSF) eosinophils collected from AF heterozygous (nu/+) mice infected with Angiostrongylus cantonensis (A.c) for 20 days or peritoneal eosinophils harvested from the peritoneal cavity of nu/+ mice infected with Mesocestoides corti (M.c) for 20 days were iv rtansferred into uninfected, normal nu/nu mice or into those infected with A.c for 17 days. Eosinophil accumulation in CSF occurred in only A.c-infected nu/nu mice transferred with eosinophils. A.c-infected nu/nu mice with CSF eosinophilia showed significantly low intracranial worm recovery. A combined injection of spleen cells from A.c-infected nu/+ mice along with CSF eosinophils caused the suppression of eosinophil accumulation in the CSF of A.c-infected nu/nu mice. Transfers of fluorochrome-labeled eosinophils from CSF or peritoneal cavity indicated that almost all eosinophils accumulated in the CSF of the recipient nu/nu mice were transferred-eosinophils themselves. These data suggest that the pre-existence of intracranial worms in the recipient mice is a prersquisite for CSF eosinophil accumulation and also that CSF eosinophilia is involved in the killing of intracranial worms. 2. Eosinophil accumulation in permissive host (rats) : Intraventricular injection of A.c-young adult worm (YA) antigen, A.c-egg antigen, or M.c antigen was performed in rats with peripheral eosinophilia due to (1)A.c infection for 40-43 days, (2) M.c infection for 20-23 days and (3) A.c-adult worm infection for 22-23 days following intrapulmonary transfers of YA.Monitoring of CSF eosinophilia in these rat groups indicated that CSF eosinophil accumulation occurred in A.c-infected rats following injection with A.c-egg or M.c antigen but not with YA antigen. These findings coincide with in vitro chemotactic activity of A.c-egg and M.c antigens against rat-eosinophils. It seems likely that the induction of eosinophi
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