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IN VITRO CULTIVATION OF ANGIOSTRONGYLUS

Research Project

Project/Area Number 04670225
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionCHIBA UNIVERSITY

Principal Investigator

HATA Hidekazu  CHIBA UNIVERSITY SCHOOL OF MEDICINE, ASSISTANT, 医学部, 助手 (00110304)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Masashi  CHIBA UNIVERSITY SCHOOL OF MEDICINE, ASSISTANT, 医学部, 助手 (80009654)
NIIMURA Munetoshi  CHIBA UNIVERSITY SCHOOL OF MEDICINE, ASSISTANT, 医学部, 助教授 (60059095)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsin vitro culture / Angiostrongylus cantonensis / Angiostrongylus costaricensis / nutritional requirement / essential amino acids
Research Abstract

The purpose of this research project is to establish the cultivation methods for Genus Angiostrongylus. Recently, we successfully cultured A.costaricensis(Ac) from the third stage larvae to young adults in chemically defined medium. Nutritional requirements of Ac were examined by deletion of single components from this defined medium. Ten amino acids, choline cloride and glucose were shown to be essential for their development. This is the first study to identify the essential nutrients for development of mammalian parasitic nematodes. The essential component in red blood cells (RBCs), which was essential nutrients for the development of young adult worms of Ac, was examined, and although the investigations were now continued, at least, hemoglobin free ghost and hemin did not support their development. On the other hand, in the cultivations of A.cantonensis, the third stage larvae were cultured to the late third stage, and the 4th stage larvae recovered from rat brain successfully cultured to young adults in same defined medium successfully cultured Ac. Using this culture system, drug screening was performed against the third stage larvae of A.cantonensis, ammnonium salt of embelin, one of the crude drug, were found to have strong antiparasitic effects. In the course of the development of Ac in chemically defined medium, the metabolites were collected, purified the proteins from the metabolites and examined for their antigenic specificity against Ac. However, we could not get the result within the research term. These investigations are now continued.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] HATA H.: "In vitro cultivation of the third stage and fourth stage larvae of Angiostrongylus costaricensis" Journal of Veterinary Medical Science. 55. 343-347 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] HATA H.: "Essential amino acids and other essential components for the development of Angiostrongylus costaricensis from third stage larvae to young adults" Journal of Parasitology. (accepted). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hata, H.: "In vitro cultivation of the third and fourth stage larvae of Angiostrongylus cantonensis." J.Vet.Med.Sci.55. 345-347 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hata, H.: "Essential amino acids and other essential components for the development of Angiostrongylus costaricensis." J.Parasitol.(accepted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] HATA H.: "In vitro cultivation of the third stage and fourth stage larvae of Angiostrongylus costaricensis" Journal of Veterinary Medical Science. 55(2). 343-347 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] HATA H.: "Essential amino acids and other essential components for the development of Angiostrongylus costaricensis from third stage larvae to young adults" Journal of Parasitology. (accepted). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] HATA H.: "In vitro cultivation of the third and fourth stage larvae of Angiostrongylus cantonensis" Journal of Veterinary Medical Science. 55(2). (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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