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Control of genomic RNA replication by the nonstructural protein NS2 of influenza A virus.

Research Project

Project/Area Number 04670279
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionJichi Medical School

Principal Investigator

ODAGIRI Takato  Jichi Medical School, Lecturer, 医学部, 講師 (80177237)

Co-Investigator(Kenkyū-buntansha) TANAKA Toshinori  Jichi Medical School, Lecturer, 医学部, 講師 (30146154)
TASHIRO Masato  Jichi Medical School, Associate Prof., 医学部, 助教授 (90111343)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsInfluenza A virus / Nonstructural NS2 / Replication, transcription / DI particle / RNA polymerase / インフルエンザウイルスA / RNAの転写の複製 / インフルエンザウイルスA型 / RNAの転写,複製 / RNAポリメラーゼ遺伝子
Research Abstract

The genome RNA segment 8 of influenza A virus encodes two nonstructural proteins, NS1 and NS2. Function of these proteins, in particular for NS2 protein, in viral replication cycle is unknown. We have succeeded to isolate an NS2 mutant. Wa-182, which produced a large amount of defective interfering(DI) particles lacking the PA polymerase gene. In the present study using the NS2 mutant Wa-182 and wild type viruses whose NS2 protein was expressed either at the beginning of infection or at the late phase of infection, we demonstrated (1) that NS2 protein promoted normal replication of genome RNA segments through the suppression of DI-RNA synthesis, (2) that NS2 protein conferred selective packaging of genome RNAs by an intermolecular recognition among RNA segments, and (3) that NS2 protein controlled the initiation of genome RNA replication by the regulation of cRNA synthesis. These observations indicate that the NS2 protein functions to be a regulator in multiple steps of viral growth cycle.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Takato Odagiri: "An amino acid change in the NS2 protein of an influenza A virus mutant causes generation of defective interfering(DI) particles by amplifying DI RNAs…" J.Gen.Virol.75. 43-53 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takato Odagiri: "Reversed misreading of a GC doublet by the modified T7 DNA polymerase,Sequenase." Virus Genes. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masashi Urabe: "Persistence of viral genes in a variant of MDCK cells after productive replication of a mutant of influenza virus A/WSN." Arch.Virol.128. 97-110 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takato Odagiri: "Structure and function of the influenza virus genome." Protein,Nucleic Acid,Enzyme. 37. 96-102 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takato Odagiri: "Synthesis of the NS2 nonstructural protein messenger RNA of influenza A viruses occurs in the absence of viral protein synthesis." Arch.Virol.120. 281-288 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takato Odagiri: "Structure and function of the NS gene of influenza viruses." Med.Rev.36. 77-106 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takato Odagiri: "An amino acid change in the NS2 protein of an influenza A virus mutant causes generatation of defective interfering(DI) particles by amplifying DI RNAs and suppressing complementary RNA synthesis." J.Gen.Virol.75. 43-53 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takato Odagiri: "Reversed misreading of a GC doublet by the modified T7 DNA polymerase, Sequenase." Virus Genes. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masashi Urabe: "Persistence of viral genes in a variant of MDCK cell after productive replication of a mutant of influenza virus A/WSN." Arch.Virol.128. 97-110 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takato Odagiri: "Structure and function of the influenza virus genome." Protein, Nucleic Acid Enzyme. 37. 96-102 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takato Odagiri: "Synthesis of the NS2 nonstructural protein messenger RNA of influenza A viruses occurs in the absence of viral protein synthesis." Arch.Virol.120. 281-288 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takato Odagiri: "Structure and function of the NS gene of influenza viruses." Medical Rev.36. 77-106 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takato Odagiri: "Mutation in NS2, a nonstructural protein of influenza A virus, extragenically causes aberrant replication and expression of the PA gene and leads to generation of defective interfering particles." Proc.Natl.Acad.Sci.USA. 87. 5988-5992 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takato Odagiri: "Nucleotide sequence of the PA gene of influenza A/WSN/33(H1N1)." Nucleic Acid Res.18. 594 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kiyotake Tobita: "Nucleotide sequence and some biological properties of the NS gene of a newly isolated influenza B virus mutant which has a long carboxyl terminal deletion in the NS1 protein." Virology. 174. 314-319 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takato Odagiri: "An amino acid change in the NS2 protein of influenza A virus mutant causes generation of defective interfering(DI) particles by amplirying DI RNAs and" J.Gen.Virol.75. 43-53 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takato Odagiri: "Reversed misreading of a GC doublet by the modified T7 DNA polymerase,Sequenase." Virus Genes. (in press ). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masashi Urabe: "Persistence of viral genes in a variant of MDCK cell after productive replica-tion of a mutant of influenza virus A/WSN." Arch,Virol.128. 97-110 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takato Odagiri: "Structure and function of the influenza virus genome." Protein,Nuleic Acid,Enzyme. 37. 96-102 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takato Odagiri: "Synthesis of the NS2 nonstructural protein messenger RNA of intluenza A viruses occurs in the absence of viral proteinsynthesis." Arch.Virol.120. 281-228 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Taato Odagiri: "Strucuture and functions of the NS gene of influenza viruses." Med.Rev.36. 77-106 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takato Odagiri: "Mutation in NS2,a nonstructural protein of influenza A virus, extragenically causes aberrant replication and expressiOn of the PA gene and leads to generation of defective interfering particles." Proc.Nat1.Acad.Sci.USA. 87. 5988-5992 (1990)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takato Odagiri: "Synthesis of the NS2 nonstructural protein messenger RNA of influenza A viruses occurs in the absence of viral protein synthesis." Arch.Viro1.120. 281-288 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takato Odagiri: "Structure and function of the NS gene of influenza A viruses." Medical Rev. Minophagen. 36. 141-147 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takato Odagiri: "Structure and function of the influenza virus gene." Protein,Nucleic acid and enzyme. 37. 1279-1285 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takato Odagiri: "Amino acid change in the NS2 protein of an influenza A virus mutant causes generation of defective interfering (DI)particles by amplifying DI RNAs and suppressing complementary RNA synthesis." Virology. (1993)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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