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Analysis of a component associated with DNA polymerase of human cytomegalovirus

Research Project

Project/Area Number 04670280
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionTokai Univertsity

Principal Investigator

IHARA Seiji  Tokai Univ.Sch.of Medicine, Associate Professor, 医学部, 助教授 (50096202)

Co-Investigator(Kenkyū-buntansha) TAKAKOSHI Masataka  Tokai Univ.Sch.of Medicine, Lecturer, 医学部, 講師 (80221373)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1992: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsHuman cytomegalovirus / DNA polymerase / UL53 / Temperature-sensitive mutant / Towne株
Research Abstract

The human cytomegalovirus (strain Towne) temperature-sensitive mutant ts256 exhibits a virus sepecific DNA polymerase-negative phenotype. The position of the mutation of ts256 was determined by three step marker-rescue assays to be within a 5.1 kb Xbal-BamHl fragment between map unit 0.33 and 0.35 in a HindIIl-D fragment located in a long unique region. Nucleotide sequencing showed that the 5.1 kb fragment contained three openreading frames corresponding to those of the genes for UL52, UL53 and Ul54 (DNA polymerase gene), respectively, of strain AD169. The functions of UL52 and UL53 are unknown. Comparison of the DNA sequences of the 5.1 kb fragments of the wild-type and ts256 mutant revealed two base changes within UL53 and UL54, respectively, which result in amino acid substitutions. The mutation in the UL54 gene was located within a distinct conserved region VI common to alpha-like DNA polymerases, suggesting that this base change would be responsible for DNA negative phenotype.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Masataka,Takeshoshi: "Inducible expression of a foreign gene inserted into the human cytomegalovirus genome." J.Gen.Virol.74. 1649-1652 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takakoshi Masataka et al.: "Induvible expression of a foreign gene inserted into the human cytomegalovirus genome." J.Gen.Viral.74. 1649-1652 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Masataka,Takekoshi: "Inducsble expression of a forign gene inserted into the human cytomegalovirus genome." J.Gen.Vivol.74. 1649-1652 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Masataka Takekoshi: "site-specific insertion into the human cytomegalovirus genome of a foreign gene under control of the SV40 promoter" Gene. 101. 209-213 (1991)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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