Study on the Genetic Polymorpphisms of Serum Proteins from Protease-treated Samples and its Application to Forensic Medicine.
| Project/Area Number |
04670355
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Ehime University |
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Principal Investigator |
NISHIMUKAI Hiroaki Ehime Univ.School of Med.Associate Prof., 医学部, 助教授 (00079758)
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| Co-Investigator(Kenkyū-buntansha) |
SHINOMIYA Kaoru Ehime Univ.School of Med.Lecturer, 医学部, 講師 (90035759)
SHINOMIYA Takaaki Ehime Univ.School of Med.Professor, 医学部, 教授 (30035406)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Protease / Sialidase / Electrophoresis / Isoelectric Focusing / Serum Proteins / Genetic Polymorphism / アロタイプ / 血漿蛋白質 / 補体系蛋白質 / 凝固線溶系因子 |
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| Research Abstract |
We studied the genetic polymorphisms of serum proteins by using electrophoresis, isoelectric focusing and SDS-PAGE.Samples for the typings were pre-treated with protease(carboxypeptidase B, carboxypeptidase Y, papain, V8-protease, etc.) and sialidase. Patterns after electrophoresis were visualized by electroblotting or immunofixation. The results obtained so far are as follows. 1. We found additional new variants of C3 in a Japanese pupulation. One variant was named as FO2 from a comparative study, and an amino acid substitution of Arg to Gln was found in the FO2 molecule. (submitted for publication). 2. C4 typing was performed successfully from plasma or serum samples. The samples were treated with both carboxypeptidase B and sialidase prior to electrophoresis. The C4 polymorphism is invaluable not only for the paternity test in medico-legal field but also for the study of etiology in clinical medicine. We examined the samples from patients with IgA-nephropathy, pemphigus, bullous pemphigoid or systemic lupus erythematosus, and found that the silent allele of Alocus(C4**AQO) was strongly associated with those diseases.(in press). 3. We found one common and four variant alleles of ATIII system in Japanese. Typing method, variants and nomenclature(alpha-numeric code) were described in our report (in press). 4. In the IEF-patterns of complement HF, many non-genetically controlled bands were observed ; the further study is required. Severa new findings have been obtained during the typings of complement BF and Cls (the results will be describled elsewhere). 5. Detection of genetic marker has been performed from bloodstain sample. The treatment of bloodstain extracts with florisil gives good results for HP and TF typings. We are now studying the determination of genetic markers from bloodstains by using the florisil-and enzyme-trated extracts as the samples of electrophoresis.
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Report
(3 results)
Research Products
(6 results)
