| Project/Area Number |
04670369
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | University of Tsukuba |
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Principal Investigator |
YAMANE Kazuhide University of Tsukuba, Institute of Clinical Medicine, Associate Professor, 臨床医学系, 助教授 (60111390)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Hiroshi University of Tsukuba, Institute of Clinical Medicine, Assistant Professor, 臨床医学系, 講師 (00179243)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Endothelin / Systemic sclerosis / Raynaud's phenomenon / Inflammatory reaction / Polymorphonuclear leukocyte / Endothelial cell / Monocyte / Leukotriene / 付着反応 / 白血球 / DLco |
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| Research Abstract |
1.Significance of endothelin-1 in clinical picture of systemic sclerosis.
Endothelin-1(ET-1) is a novel potent vasoconstrictive peptide discovered in the supernatant fraction of cultured endohelial cells. We measured plasma levels of ET-1 using a sensitive sandwich enzyme immunoassay. Plasma levels of ET-1 in paitents with systemic sclerosis were higher than those in normal subjects. Patients with diffuse scleroderma had higher levels of ET-1 compared with those with limited scleroderma. Plasma ET-1 levels correlated inversely with carbon monoxide diffusing capacity. Measurement of plasma ET-1 leves may be useful as a predictor of prognosis of systemic sclreosis.
2.Significance of ET-1 in inflammatory reaction of systemic sclerosis.
We examined an effect by ET-1 on leukotriene B _4 release from monocytes and polymorphonuclear leukocytes. We found that ET-1 augmented leukotriene B _4 release from monocytes and polymorphonuclear leukocytes in normal subjects. This augmenting effect by ET-1 was decreaed in patients with systemic sclerosis. We further studied an effect by leukotriene B _4 and ET-1 on polymorphonuclear leukocyte-endothelial adhesion. While leukotriene B _4 increased this adhesive reaction using polymorphonuclear leukocytes from normal subject and patients with systemic sclerosis, ET-1 showed no effect on adhesive reaction using cells from normal subjects and patients with systemic sclerosis.
Although plasma levels of ET-1 were elevated in patients with systemic sclerosis, there was no evidence that ET-1 was involved directly in inflammatory reaction in systemic sclerosis.
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