• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effect of Selective Brain Hypothermia on Regional Cerebral Blood Flow, Brain Tissue Metabolites and Neurotransmitter Release in Spontaneously Hypertensive Rats

Research Project

Project/Area Number 04670390
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

IBAYASHI Setsuro  Kyushu Univ., Dept.Int.Med., Assistant Professor, 医学部, 助手 (40213201)

Co-Investigator(Kenkyū-buntansha) 高野 健太郎  国立療養所, 福岡東病院・内科, 厚生技官
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
KeywordsBrain temperature / Cerebral ischemia / Spontaneously hypertensive rats / Brain tissue metabolism / Neurotransmitter / Neuronal death / 脳血流 / 脳代謝 / 脳血流量 / 脳織織代謝 / 脳温度勾配 / 脳温可変装置
Research Abstract

To investigate the effect of selective hypothermia on regional cerebral blood flow (rCBF), brain tissue metabolism and neurotransmitter release in rats, we newly developed a brain thermoregulator for small animals. In the first year, regional brain temperature and rCBF were simultaneously measured using a Teflon-coated combined platinum electrode and thermocouple probe inserted into the parietal cortex and the thalamus in spontaneously hypertensive rats (SHR). In the second year, one hour of forebrain ischemia was induced by bilateral carotid artery ligation, and the concentrations of brain tissue metabolites were measured enzymatically. The difference in regional brain temperature between the cortex and the thalamus became greater when cortical temperature was set to the lower level such as 30゚C than 37゚C.In the rats induced selective brain hypothermia (30゚C) immediately after the initiation of ischemia, the level of adenosine triphosphate (ATP) was significantly higher than that in n … More ormothermic rats (36゚C). Our study suggests 1) the newly developed brain thermo-regulator is useful in the small animal experiments, 2) regional brain temperature regulates regional cerebral blood flow, and 3) selective brain hypothermia is effective and cytoprotective in the treatment of brain ischemia.
In the final year, we examined whether brain hypothermia modulates the release of excitatory or inhibitory amino acids during cerebral ischemia in the hippocampus of aged SHR using a microdialysis technique. Hippocampal temperature was maintained 36゚C (normothermia), 33゚C (mild hypothermia) or 30゚C (moderate hypothermia), and rectal temperature 37゚C in this study. During cerebral ischemia, concentrations of glutamate, aspartate and glycine significantly increased 6-, 5-and 2-fold the resting values, respectively, in the normothermic rats, whereas hypothermia markedly inhibited the rise to 220,110 and 80% of the resting values. Similarly the elevation of taurine was 16-fold in the normothermic rats and significantly attenuated to 3-to 10-fold in both moderately and mildly hypothermic rats. The hypothermia also attenuated the ischemia-induced elevation of GABA.Histopathological grading of ischemic damage in the hippocampal CA1 areas after 7 days was significantly ameliorated in the moderate hypothermic rats (0.5 <plus-minus> 0.4) compared with the normothermic ones (1.8 <plus-minus> 0.3). These results suggest that brain hypothermia protects the neurons against ischemic insult in the hippocampus of aged SHR,and it may be, at least in part, attributed to the preservation of synaptic homeostasis preventing the excessive release of both excitatory and inhibitory amino ac Less

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi