| Project/Area Number |
04670426
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
KOYAMA Yasuo Nagoya University, School of Medicine, Instructor, 医学部, 助手 (40192067)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Yoshihide Nagoya Insitute of Techonology, Health adiministration Center, Asistant Professo, 保健管理センター, 講師 (40181284)
IMOTO Masami Nagoya University, School of Medicine, Asistant Professor, 医学部, 講師 (10158769)
井本 正巳 名古屋大学, 医学部, 講師 (10158769 )
|
|---|
| Project Period (FY) |
1992 – 1993
|
| Project Status |
Completed (Fiscal Year 1993)
|
| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
|
|---|
| Keywords | Adhesion Molecule / Lymphoid follicle / Dendritic Cell / Chronic Hepatitis / Liver Cirrhosis / Hepatocellular Caricinoma / Immunohistochemistry |
|---|
| Research Abstract |
Liver specimens with chronic hepatitis (CH), liver cirrhosis (LC) and hepatocellular cqrcinoma (HCC) were examined immunohistochemically. For the control study, normal liver specimens were also examined. Serum intercellular adhesion molecule-1 (ICAM-1) levels were determined by ELISA method among normal subjects, CH, LC and HCC.
In normal liver, expression of ICAM-1 and intercellular adhesion molecule-2 (ICAM-2) was restricted to sinusoidal lining cells and portal vascular endothelial cells. In CH and LC, ICAM-1 and ICAM-2 were enhanced in the area of necrosis. ICAM-1 was also expressed on hepatocytes in a honeycomb patern. Follicular dendritic cells in the center of lymphoid follicles were positive for ICAM-1. These cells were in contact with lymphocyte function-associated antigen-1. In HCC, ICAM-1 and ICAM-2 were detected in carcinomatous sinusoids and carcinoma cells. The capsule and sinusoids of HCC contained lymphocytes.
In contrast to controls, serum ICAM-1 levels were significantlly elevated in CH, LC and HCC.In the cases of CH, there was a significantcorrelation between the levels of ICAM-1 and ALT.The mean ICAM-1 level in HCC cases, no significant correlation was observed between ICAM-1 and tumor size or tumor markers (AFP and PIVKA-II).
|
