| Project/Area Number |
04670443
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Juntendo University School of Medicine |
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Principal Investigator |
WATANABE Sumio Juntendo University, Gastroenterology, Lecturer, 医学部, 講師 (20138225)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOI Yukio Juntendo University, Gastroenterology, Lecturer, 医学部, 講師 (20150633)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | liver transplantation / bile / microcirculation / Ito cell / Kupffer cell / calcium / cytoskeleton / eudothelin / 肝移植 / 胆汁 / 微小循環 / 伊東細胞 / クッパー細胞 / カルシウム / 細胞骨格 / エンドセリン / 毛細胆管 / 胆汁分泌 |
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| Research Abstract |
The bile formation is well inferenced by hepatic microcirculation, parenchymal cell function and bile canalicular function. Re-perfusion injury which is commonly occurred in liver transplantation and the mechanism or re-perfusion injury is still unknown. Accumulated data strongly suspected that damaged hepatic microcirculation plays a role on this process. In this project, we investigated the role of Ito cells and Kupffer cells on hepatic microcirculation and revealed that Ito cells had contractile activity and modulated hepatic blood flow. And this contratile activity was controlled by actin-myosin system. Kupffer cells had phagocytic activity which was also controlled by the cytoskeletal system and blocked hepatic blood flow. Therefore, the maintenance of the function of these cells was important for the success of liver transplantation.
In this study, the role of calcium in parenchymal hepatocytes was also investigated and revealed that intracellular calcium playd an important role on bile formation and liver regeneration.
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