| Project/Area Number |
04670445
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | The JIKEI University School of Medicine |
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Principal Investigator |
ZENIYA Mikio The Jikei University School of Medicine Lecturer, 医学部, 講師 (70138767)
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| Co-Investigator(Kenkyū-buntansha) |
TODA Gotaro The Jikei University School of Medicine Professor, 医学部, 教授 (40090500)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Autoimmune hepatitis / Asialoglycoprotein receptor / T cell receptor / T cell receptor gene subfamily(Vbeta) / annexin / 漫性肝炎 / 免疫学的肝障害 |
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| Research Abstract |
Asialoglycoprotein receptor(ASGPR), hepatic lectin, is known as the liver specific glycoprotein. Previous reports suggested that this glycoprotein might be one of the candidate on immune-mediated liver cell injury especially in autoimmune hepatitis (AIH).
Is this study, to clarify the immune-mechnism of liver cell damage, we tried to establish the assay system of antibody to ASGPR, and analyze the pathophisiology of this antibody in the course of chronic hepatitis. ASGPR was purified from human liver obtained from autopsied samples. A piece of liver was homogenated and separated by affinity Sepharose 4Bcolumn conjugated orthomucoid which has specific binding activity to ASGPR.The purified sample was tested by PAGE, and provided for the assay of anti-ASGPR antibody by western blotting. However immunoblotting analysis was not successful because of the existence of severe smear results. We made synthesized peptides of ASGPR and examined the proliferative activity of peripheral lymphocyte against them. These resulted poor responses, indicating the possibility that there was no ASGPR-specific T lymphocytes in peripheral lymphocytes. We studied TCR V beta repatore of peripheral and liver-infiltrated lymphocytes and it became clear that the TCR V beta repatore of liverinfiltrating lymphocytes was different from that of peripheral lymphocytes.
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