The role of cytokines including IL-10 in bronchial asthma

• Project/Area Number: 04670453
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Tohoku University
• Principal Investigator: OKAYAMA Hiroshi Tohoku Univ. First Department of Internal Medicine, School of Medicine, Assistant Proffessor, 医学部, 助手 (10160730)
• Co-Investigator(Kenkyū-buntansha): TAMURA Gen Tohoku Univ. First Department of Internal Medicine, School of Medicine, Assistan, 医学部, 助手 (70188431) ; SHIMURA Sanae Tohoku Univ. First Department of Internal Medicine, School of Medicine, Assistan, 医学部・付属病院, 講師 (20154312)
• Project Period (FY): 1992 – 1993
• Project Status: Completed (Fiscal Year 1993)
• Budget Amount: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: IL-5 / IL-10 / glucocorticoid / human / monomuclear cell / gene expression / atopy / asthma / ステロイド / 喘息

Research Abstract

The effect of glucocorticoids on interleukin 5 induction and its mechanism from normal and atopic human mononuclear cells
The effect of glucocorticoid, dexamethasone on IL-10 gene expression by human nonocytes, lymmphocytes and peripheral blood mononuclear cells (PBMC) from five healthy volunteers was examined. PBMC expressed IL-10 gene without stimulation and increased by PHA or LPS stimulation, which was suppressed by dexamethasone to 50%. The results indicate that IL-2, IL-4 and IL-5.
The results suggest that the regulation of IL-10 gene expression by dexamethasone is different from that of many cytokines. IL-10 may play an important role in the cytokine regulation of alloegie inflammation during glucocorticoid therapy.

Research Products

• [Publications] Hiroshi Okayama et al.: "Glucocorticoids supressed production and gene expression of interleukin-5 by peripheral blood mononuclear cells in atopic patients and normal subjects" J.Allergy and Clinical Immunology. 93(in press). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 岡山博 他8名: "グルココルチコイドによるインターロイキン5遺伝子発現と産生抑制作用" 呼吸. 14(印刷中). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fushimi T.,Okayama H.,et al: "The effect of Dexamethasone on IL-10 gene expression by human lymphocyte and monocyte" Respiratory and Critical Care Medicine Am Rev Respir Dis. 149(in press). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hiroshi Okayama et al: "Glucocorticoids supressed production and gene expression of interleukin-5 by peripheral balood mononuclear cells in atopic patients and normal subjects" J Allergy Clin Immunol. Vol 93. (in press) (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hiroshi Okayama et al: "Supressive effect of glucocorticoids on interleukin-5 induction and its mechanism from human peripherial mononuclear cells (in Japanese)" Kokyu. vol 14. (in press) (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Toshiaki Fushimi, Hiroshi Okayama et al: "The effect of dexamethasone on IL-10 gene expression by human lymphocytes and monocytes" American Journal of Respiratory Celll and Molecular Biology. 10. (in press) (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hiroshi Okayama et al.: "Glucocorticoids supressed production and gene expression of interleukin-5 by peripheral blood mononuclear cells in atopic patients and normal subjects" J.Allergy and Clinical Immvnology. (in press). (1994)
• [Publications] 岡山 博 他8名: "グルココルチコイドによるインターロイキン5遺伝子発現と産生抑制作用" 呼吸. (印刷中). (1994)
• [Publications] Fushimi T.,Okayama H.,et al: "The effect of Dexamethasone on IL-10 gene expression by human lymphocyte and monocyte" Am Rev Respir Dis. (in press). (1994)
• [Publications] T.Fushimi H.Okayama,et al.: "Dexamethasone Supresses Both IL-5 Production and Its Gene Expression by Human PBMC" Am Rev.Resp.Disea.(1993)