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Changes in Opioid and Ach of the Rat Brain during Resistive Loaded Breathing

Research Project

Project/Area Number 04670456
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionTohoku University

Principal Investigator

KIKUCHI Yoshihiro  Tohoku University, First department of Internal Medicine, Assistant Professor, 医学部・附属病院, 助手 (20195217)

Co-Investigator(Kenkyū-buntansha) YAMAUCHI Kouhei  Tohoku University, School of Medicine, Assistant Professor, 医学部, 助手 (20200579)
HIDA Wataru  Tohoku University, School of Medicine, Assistant Professor, 医学部, 講師 (10142944)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsEnkephaline / Acetylcholine / Microdialysis / In situ Hybridyzation / Control of breathing / Hypoxic ventilatory response / Hypercapnis / 呼吸困難
Research Abstract

(1) We have examined changes of mRNA for preproenkephaline (PPE)-A, which is a precursor of enkephaline, in the rats cerebral cortex during chronic resistive loaded breathing. Northern blot revealed that PPE-A mRNA was induced after 3 days of airway stenosis, and the induction continued for at least 2 weeks. In situ hybridization revealed that PPE-A was gradually induced in frontal cortex. These results suggest that the endogenous opioid system may be at work as an important compensatory mechanism to reduce the reaspiratory sensation during chronic respiratory stress.
(2) We have investigated acetylcholine (Ach) release in the hypocampus, cingulate cortex or hypothalamus of rat brain during hypercapnia or hypoxia. 10% CO_2 induced Ach in the hypocampus and hypothalamus by 30-100%, but it did not induced in the striatum. Hypoxia increased Ach in the same area, but the degree was much smaller than that of hypercapnia.
(3) We measured glutamate (Glu) release in the nucleus tractus solitarius of rats brain during hypoxia by using in vivo microdialysis. Glutamate increased during hypoxia or during doxapram infusion in carotid body intact rats, but it did not increased in rats with bilateral carotid body resection. Microinjected glutamate into the NTS increased ventilation. MK801, a NMDA receptor antagonist, perfused in the NTS blocked the ventilatory increase during hypoxic ecposure. These results suggests that glutamate may be a neurotransmitter in the NTS in response to peripheral chemoreceptor activation during hypoxia.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Y.Kikuchi: "Chemosensitivity and perception of dyspnea in patients with life-threatening attacks of asthma" The New England Journal of Medicine. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Mizusawa: "Role of glutamate as the neurotransmitter in the nucleus tractus solitarius during hypoxia" Journal of Physiology(London). (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] M.Aoki: "Preproenkephalin gene expression in the rat cerebral cortex during chronic tracheal stenosis" Neurological Research. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] W.Hida: "Prevalence of sleep apnea among Japanese industrial workers determined by a portable sleep monitoring system" Respiration. 60. 332-337 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Okabe: "Role of chemical drive in recruiting upper airway and inspiratory muscles in patients with sleep apnea." American Review of Respiratory Disease. 147. 190-195 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Kikuchi: "Effects of changes in breathing pattern on the sensation of dyspnea during inspiratory loaded breathing" Control of Breathing and Its Modeling Perspective. 405-408 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Kikuchi: "Chemosensitivity and perception of dyspnea in patients with life-threatening attacks of asthma" The New England Journal of Medicine. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Mizusawa: "Role of glutamate as the neurotransmitter in the necleus tractus solitarius during hypoxia" Journal of Physiology (London). (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] A.Aoki: "Preproenkephalin gene expression in the rat cerebral cortex during chronic tracheal stenosis" Neurological Research. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] W.Hida: "Prevalence of sleep apnea among Japanese industrial workers determined by a portable sleep monitoring system" Respiration. Vol.60. 332-337 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] S.Okabe: "Role of chemical drive in recruiting upper airway and inspiratory muscles in patients with sleep apnea" American Review of Respiratory Diseas. Vol.147. 190-195 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Kikuchi: "Effects of changes in breathing pattern on the sensation of dysphea during loaded breathing" Control of Breathing and Its Modeling Perspective. 405-408 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Y.Kikuchi et al.: "Chemosensitivity and perieption of dysprea in patients with life-threatening attacks of asthma." N.Engl.J.Med.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] A.Mizusawa et al.: "Role of glutamate as the neurotransmutter in the muclaus tractus solitarius during hypoxia" J.Physiol.(London). (in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] W.Hida et al.: "Prevalence of sleep apnea among Japanese inductrial workers determined by a portable sleep monitoring system" Respiration. 60. 332-337 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Satoh et al.: "Effect of posture on CO_2 response in patients with sleep apnoea" Thorax. 48. 537-541 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Okabe et al.: "Role of chemical drive in recruiting upper airway and inspiratory muscles in patients with sleep apnea." Am.Rev.,Respir.Dis.147. 190-195 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Y.Kikuchi et al.: "Effects of chenges in breathing pattern on the seusation of dysprea during inspiratory loaded breathing." Control of breathing and its modeling perspective. 405-408 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] O.Taguchi: "Prostaglandin E_2 inhalation increase the sensstion of dysprea during exercise" Am Rev Respin Dis. 145. 1346-1349 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Miki: "Effects of pharyngeal lubrication on the opening of obstructive uppear airway" J.Appl Physiol. 72. 2311-2316 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] FD McCool: "Effects of fatigue on matimel inspirotory pressure flow capacity" J.Appl Physiol. 73. 36-43 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] W.Hida: "P wave height during incremental exercise in patients with chronic anwy obstruction" chest. 102. 23-30 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] N.Iwase: "Effects of repetitire airway obstruction on O_2 sataration systemic and pulmonary pressure in anesthetized dogs" Anr Rev Respoir Pis. 146. 1402-1410 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] C.Miura: "Effects of posture on flow volume curves during normocapnia and hypocapnia in patients with obstructive sleep apnea" Thorax. 47. 524-528 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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