| Project/Area Number |
04670505
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Neurology
|
|---|
| Research Institution | Kurume University |
|---|
Principal Investigator |
SHOJI Hiroshi Kurume Univ., Medichine, Prof., 医学部, 教授 (90122621)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HONDO Ryo Inst.Pub.Health, Microbiol., Chief, 衛生微生物学部, 室長 (20114639)
YAMAMOTO Shigeru Kurume Univ., Medicine, Assist.Prof., 医学部, 助教授 (10080607)
AYABE Mitsuyoshi Kurume Univ., Medicine, Assist., 医学部, 助手 (40232003)
|
|---|
| Project Period (FY) |
1992 – 1993
|
| Project Status |
Completed (Fiscal Year 1993)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Keywords | Herpes simplex virus / Hybridization / Polymerase chain reaction / Herpes simplex encephalitis / Herpes simplex meningitis |
|---|
| Research Abstract |
Diferentiation of herpes simplex virus (HSV) types 1 and 2 in cerebrospinal fluid (CSF) of 17 patients with serologically diagnosed HSV encephalitis and meningitis or acute limbic encephalitis was determined by stringent hybridization of ploymerase chain reaction (PCR)-amplified DNAs. Ten of 17 patients were positive ; six with HSV 1 isolates and four with HSV 2 isolates. We detected HSV type 1 in two cases of meningitis, although meningitis is generally thought to be caused by type 2. Additionally, HSV type 2 was found in one case of acute adult encephalitis, generally due to HSV type 1. HSV DNAs could be detected for over one month after onset, although our cases contained several prolonged and recurrent cases. HSV DNA genomes were not detected in three cases of acute limbic encephalitis. Our study indicates that this method can be used for type differentiation in HSV CNS infections.
|
