• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functions of proteoglycans during the development of nervous tissues.

Research Project

Project/Area Number 04670509
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Neurology
Research InstitutionInstitute for Developmental Research, Aichi Human Service Center

Principal Investigator

KATOH Ritsuko  Department of Perinatology, Institute for Developmental Research, Aichi Prefecture Colony, Room Chief., 周正期学部, 室長 (80100163)

Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
KeywordsProteoglycans / PC12 cells / Dorsal root ganglia / Nerve growth factor / Proliferation / Neurite outgrowth / Laminin / Immunocytochemistry / 軸索形成 / コンドロイチン硫酸 / ラット / 細胞周期 / グリコサミノグリカン
Research Abstract

To elucidate functions of chondroitin sulfate proteoglycans (CSPGs) derived from the rat brain, we investigated the effects of brain CSPGs on proliferation and/or neurite outgrowth of PC12D cells and neurons of the dorsal root ganglia (DRG) and their immunocytochemical localization in rat embryo. Brain-type CSPGs prevented increases in the number of PC12D cells by blocking the cell cycle at the G2 phase and neurite elongation from PC12D cells, DRG explants and DRG neurons. DRG was formed at around embryonic day 12.5 (E12.5) and elongated their axons toward the neural tube at around E13.5. Neurocan, one of major brain CSPGs, was located at the boundary cap, apart from sites where the afferent axons from the DRG seemed to reach the neural tube, and the roof plate, a glial barrier in axon elongation, of the spinal cord at E13.5. Neurocan has been detected around the boundary cap at E12.5 when the DRG does not elongate their axons yet. Thus, neurocan may function as a barrier molecule in axon elongation from the DRG.Another CSPG,6B4 proteoglycanlike immunoreactivity, was found at several ganglions in the peripheral nervous system (PNS), when their neurons were still proliferating. Since its antibody prevent the inhibition of proliferation of PC12D cells by 6B4 proteoglycans, this proteoglycan may influence the cell proliferation in PNS.

Report

(4 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Shozuhara,H.: "Temporal profiles of nerve growth factor β-subunit level in rat brain regions after transient ischemia." Journal of Neurochemistry. 59. 175-180 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba,R.: "Nerve growth factor-induced changes in the structure of sulfated proteoglycans in PC12 pheochromocytoma cells." Journal of Neurochemistry. 59. 282-289 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba,R.: "Core proteins of soluble chondroitin sulfate proteoglycans purified from the rat brain block the cell cycle of PC12D cells." Journal of Cellular Physiology. 156. 17-23 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba,R.: "An accerelation of age-related increases in levels of the β-subunit of nerve growth factor in selected tissues from senescence-accelerated mice(SAM-P/8)." Journal of Molecular Neuroscience. 4. 107-115 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ariga,T.: "Activation of UDP-galactose:globotriaosylceramide α1-3-galactosyltransferase during PC12D cell differentiation induced by galactosylceramide." Biochemistry. 32. 7904-7908 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yu,R.K.: "Differential effects of glycosphingolipids on protein kinase C activities in PC12D pheochromocytoma cells." Journal of Biomedical Science. 1. 229-236 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba,R.: "Regulation by androgen of levels of the β-subunit of nerve growth factor and its mRNA in selected regions of the mouse brain." Journal of Neurochemistry. 62. 2141-2147 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Oohira,A.: "Brain development and multiple molecular species pf proteoglycan." Biomedical Research. 20. 195-207 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba,R.: "Age-related change in levels of the β-subunit of nerve growth factor in selected regions of the brain: comparison between senescence-accelerated (SAM-P8) and senescence-resistant (SAM-R1) mide." Biomedical Research. 20. 251-256 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kanda,T.: "Effect of nerve growth factor and forskolin on glycosyltransferase activities and expression of a globo-series glycosphingolipid in PC12D pheochromocytoma cells." Journal of Neurochemistry. 64. 810-817 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba,R.: "Chondroitin sulfate proteoglycans in the rat brain:candidates for axon barriers of sensory neurons and the possible modification by laminin of their actions." The European Journal of Neuroscience. (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba,R.: "Two types of brain chondroitin sulfate proteoglycans: developchanges in their distribution in rat embryo and possible function." in preparation. (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba,R.: "Neurotrophic Factors(pp.185-196)" Japan Scientific Societies Press,Tokyo, 242 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shozuhara, H.et al.: "Temporal profile of beta-subunit of the nerve growth factor level in ratbrain regions after transient ischemia." J.Neurochem.59. 175-180 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba, R.et al.: "Nerve growth factor-induced changes in structure of sulfated proteoglycans in PC12 pheochromocytoma cells." J.Neurochem.59. 282-289 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba, R.and Oohira, A.: "Core proteins of soluble chondroitin sulfate proteoglycans purified from the rat brain block the cell cycle of PC12D cells." J.Cell.Physiol.156. 17-23 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katon-Semba R.et al.: "An acceleration of age-related increases in levels of the beta-subunit of nerve growth factor in selected tissues from senescence-accelerated mice (SAM-P/8)." J.Mol.Neurosci.4. 107-115 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ariga, T.et al.: "Activation of UDP-galactose : globotriaosylceramide alpha1-3-glalctosyltransferase during PC12D cell differentiation induced by galactocylceramide." Biochemistry. 32. 7904-7908 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yu, R.K.et al.: "Differential effects of glycosphingolipids on protein kinase C activity in PC12D pheochromocytoma cells." J.Biomed.Sci.1. 229-236 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba, R.et al.: "Regulation by androgen of levels of the beta-subunit of nerve growth factor and its mRNA in selected regions of the mouse brain." J.Neurochem.62. 2141-2147 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Oohira, A.et al.: "Brain development and multiple molecular species of proteoglycan." Neurosci.Res.20. 195-207 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba, R.and Kato K.: "Age-related changes in levels of the beta-subunit of nerve growth factor in selected regions of the brain : comparison between senescenceaccelerated (SAM-P8) and senescence-resistant (SAM-R1) mice." Neurosci.Res.20. 195-207 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kanda, T.et al.: "Effect of nerve growth factor and forskolin on glycosyltransferase activities and expression of a globo-series glycosphingolipid in PC12D pheochromocytoma cells." J.Neurochem.64. 810-817 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba, R.et al.: "Chondroitin sulfate proteoglycans in the rat brain : candidates for axon barriers of sensory neurons and the possible modification by laminin of their actions." Eur.J.Neurosci.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba, et al.: "Two types of brain chondroitin sulfate proteoglycans : developmental changes in their distribution in rat embryo and possible functions." (in preparation). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Katoh-Semba, R., and Oohira, A.: Neurotrophic factors (Y.Tsukada and E.Shooter, eds.), (Functional significance of proteoglycans in the morphologic differentiation of neuronal cells.). Japan Scientific Society Press, Tokyo, 185-196 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yu,R.K.: "Differential effects of glycosphingolipids on protein kinase C activities in PC12D pheochromocytoma cells." Journal of Biomedical Science. 1. 229-236 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Katoh-Semba,R.: "Regulation by androgen of levels of the β-subunit of nerve growth factor and its mRNA in selected regions of the mouse brain." Journal of Neurochemistry. 62. 2141-2147 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Oohira,A.: "Brain development and multiple molecular species pf proteo-glycan." Biomedical Research. 20. 195-207 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Katoh-Semba,R.: "Age-related changes in levels of the β-subunit of nerve growth factor in selected regions of the brain:comparison between senes-cence-accelerated (SAM-P8)and senescence-resistant(SAM-R1)mide." Biomedical Research. 20. 251-256 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kanda,T.: "Effect of nerve growth factor and forskolin on glycosyltransferase activities and expression of a globo-series glycosphingolipid in PC12D pheochromocytoma cells." Journal of Neurochemistry. 64. 810-817 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Katoh-Semba,R.: "Chondroitin sulfate proteoglycans in the rat brain:candidates for axon barriers of sensory neurons and the possible modification by 1aminin of their actions." The European Journal of Neuroscience. (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Katoh-Semba,R.: "Core proteins of soluble chondroitin sulfate proteoglycans purified from the rat brain block the cell cycle of PC12D cells" Journal of Cellular Physiology. 156. 17-23 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Katoh-Semba,R.: "An acceleration of age-related increases in levels of the b-subunit of nerve growth factor in selected tissues from senescence-accelerated mice(SCM-P/8)" Journal of Molecular Neuroscience. 4. 107-115 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ariga,T.: "Activation of UDP-galactose:globotriaosylceramide al-3-galactosyltransferase during PC12D cell differentiation induce by galactosylceramide" Biochemistry. 32. 7904-7908 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Katoh-Semba,R.: "Requlation by androgen of levels of the b-subnnit of nerve growth factor and its mRNA in selected regions of the mouse brain" Journal of Neurochemistry. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shozuhara,H.: "Temporal profiles of nerve growth factor β-subunit level in rat brain regions after transient ischemia." Journal of Neurochemistry. 59. 175-180 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Katoh-Semba,R.: "Nerve growth factor-induced changes in the structure of sulfated proteoglycans in PC12 Pheochromocytoma cells." Journal of Neurochemistry. 59. 282-289 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 仙波 りつ子: "脳可溶性コンドロイチン硫酸プロテオグリカンによるPC12細胞の増殖および突起形成阻害:細胞環境の影響" 神経化学. 31. 356-357 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Katoh-Semba,R.: "Core proteins of soluble chondroitin sulfate proteoglycans purified from ther at brain block the cell cycle of PC12D cells." Journal of Cellular Physiology. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Katoh-Semba,R.: "An Acceleration of age-related increases in levels of the β-subunit of nerve growth factor in selected tissues from senescence accelerated mice(SAM-P/8)." Journal of Molecular Neuroscience. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Katoh-Semba,R.(分担執筆): "Neurotrophic factors (pp.185-196)" Japan Scientific Societies Pree,Tokyo, 242 (1992)

    • Related Report
      1992 Annual Research Report

URL: 

Published: 1992-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi