| Project/Area Number |
04670569
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kawasaki College Of Allied Health Professions |
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Principal Investigator |
GOTO Masami Kawasaki College Of Allied Health Professions, Dept.of Applied Medical Engineering, Associate Professor, 医用電子技術科, 助教授 (50148699)
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| Co-Investigator(Kenkyū-buntansha) |
KAJIYA Fumihiko Kawasaki Medical Scool, Dept.of Mecical Engineering and Systems Cardiology, Prof, 医用工学, 教授 (70029114)
TCHIBANA Hiroyuki Kawasaki College Of Allied Health Professions, Dept.of Applied Medical Engineeri, 医用電子技術科, 助手 (00241216)
HIRAMATSU Osamu Kawasaki College Of Allied Health Professions, Dept.of Applied Medical Engineeri, 医用電子技術科, 助手 (50208849)
MOCHIZUKI Seiichi Kawasaki College Of Allied Health Professions, Dept.of Applied Medical Engineeri, 医用電子技術科, 講師 (60259596)
MATSUMOTO Takeshi Kawasaki College Of Allied Health Professions, Dept.of Applied Medical Engineeri, 医用電子技術科, 講師 (30249560)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Needle-probe video-microscope with a CCD camera / Beating heart / Subendocardial vessels / Microcirculation / Indocyanine green / FITC-dextran |
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| Research Abstract |
To understand the basis of the subendocardial vulnerability to ischemia, we evaluated the behavior of the microvessels and their blood flows in the subendocardium using a portable needle-probe video-microscope with a charge-coupled device (CCD) camera. The tip of the needle-probe was introduced into the left ventticular cavity from the left atrium of the beating canine hearts and observed the subendocardial arterioles and venules. To accentuate the small vascular images, we employed : 1) a green (complimentary color of red) filter, 2) an intracoronary injection of FITC-dextran, and 3) an intracoronary or intravenous injection of indocyanine green. Using these techniques, the arterial and venous microvessels could be identified easily, and the diameter changes could be evaluated.
We evaluated the effects of ninoglycerin on the coronary microvessels. The study showed that nitroglycerin dilated both subepicardial and subendocardial arterioles. The vasodilatory effects of nitroglycerin on the subendocardial arterioles were different depending on the sizes of the vessels. Nitroglycerin dilated larger arterioles with diameters greater than 100 mum compared to those with diameters smaller than 100 mum. Nitroglycerin increased the pulsation amplitude of the subendocardial arterioles. It was also found that nitroglycerin tended to dilate the coronary venules. Further investigation will be needed to understand the effects of nitroglycerin on the venous myocardial vessels.
Using a high-contrast blood flow marker, India ink, not only the blood flow in the myocardium but also that from the myocardium to the ventricular cavity, i.e., Thebesian venous blood flow, could be traced.
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