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Research on molecular diagnosis of viral encephalitis in children -application to early diagnosis-

Research Project

Project/Area Number 04670589
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionNagoya University

Principal Investigator

MORISHIMA Tuneo  Nagoya University School of Medicine Assistant Profassor, 医学部, 講師 (90157892)

Co-Investigator(Kenkyū-buntansha) HORIBE Keizou  Nagoya University School of Medicine Assistant Profassor, 医学部, 講師 (30209308)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
KeywordsPCR assay / Herpes simplex virus / Herpes simplex encephalitis / Cytomegalovirus / intrauterine infection / DNA / encephalitis / encephalopathy / PCR / ウイルス性脳炎 / エンテロウイルス / 髄液 / 早期診断 / ウイルスDNA / 抗ウイルス剤 / 出生前診断
Research Abstract

(1) In herpes simplex encephalitis (HSE), detection of HSV DNA in CSF of patients during acute phase was performed by means of PCR assay. By PCR,the HSV DNA was found more than 90% of HSE patients, showing that this method is useful for early diagnosis.
(2) Quantiation of HSV DNA was performed and the mode of presense of viral genome was examined by PCR method. In neonatal HSE,large quantities of HSV DNA was found in CSF as a form of infectious particles, while in HSE of older Children, the amount of viral DNA was smaller and most of the viral DNA was fragmented.
(3) In West syndrome patients, who had been infected with human cytomegalovirus (CMV) transplacentally, viral DNA was detectable in CSF,suggesting that the presense of CMV in the brain could play some roles on the progress of West syndrome.
Thus' interesting reslults were obtained during these 3 years.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Osuga T et al.: "Transfer of specific IgG and IgGsubclasses to herpes simplex virus across the blood-brain barrier and placenta in preterm and term newborns." Acta Paediatr. 81. 792-796 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kimura H et al.: "Relapse of herpes simplex encephalitis in children." Pediatr. 89. 891-894 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ando Y et al.: "Quantitative analysis of herpes simplex virus DNA in the cerebrospinal fluid of children with herpes simplex encephalitis." J Med Virol. 41. 170-173 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kimura H et al.: "T-cell receptor V a region in the cerebrospinal fluid of patients with mumps meningitis." J Med Virol. 41. 306-311 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 森島 恒雄: "単純ヘルペスウイルス" 脳と発達. 25. 124-127 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Morishima T: "New diagnostic techniques for the management of herpesviruses." HERPES. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 森島 恒雄: "新しい集中治療の指針 全身感染症(P353〜357)" 南山堂(吉矢生人、島田康弘編), 415 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 森島 恒雄: "微生物学コメンタリー ウイルス学各論(P192〜195)" 中外医学社(平松啓一,南嶋洋一編), 236 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Osuga T,Morishima T,Hanada N,Nishikawa K,Isobe K,Watanabe K.: "Transfer of specific IgG and IgG subclasses to herpes simplex virus across the blood-brain barrier and placenta in preterm and term newborns." Acta Paediatr. 81. 792-796 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kimura H,Aso K,Kuzushima K,Hanada N,Shibata M,Morishima T.: "Relapse of herpes simplex encephalitis in children." Pediatr. 89. 891-894 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ando Y,Kimura H,Miwata H,Kudo T,Shibata M,Morishima T.: "Quantitative analysis of herpes simplex virus DNA in the cerebrospinal fluid of children with herpes simplex encephalitis." J Med Virol. 41. 170-173 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kimura H,Ando Y,Shibata M,Abe T,Morishima T.: "T-cell receptor V a region in the cerebrospinal fluid of patients with mumps meningitis" J Med Virol. 41. 306-311 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Morishima T.: "Herpes simplex virus" Brain and Development. 25. 124-127 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Morishima T.: "New diagnostic techniques for the management of herpesviruses." HERPES. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Morishima T.: New treatment in ICU. Nanzando(edited by Yoshiya I and Shimada Y), 353-357 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Morishima T.: Commentary of Microbiology. Chugaiigakusha(edited by Hiramatsu K and Minamishima, 192-195 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Morishima T: "New diagnostic techniqucs for the management of herpesviruscs." HERPES. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 森島恒雄: "新しい集中治療の指針 全身感染症" 南山堂(吉矢生人,島田康弘 編), 415,(353-357) (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 森島恒雄: "微生物学コメンタリー ウイルス各論" 中外医学社(平松啓一,南嶋洋一 編), 236,(192-195) (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ando Y.et al.: "Quantitative analysis of herpes simplex virus DNA in cerebrospinal fluid of children with herpes simplex encephalitis." Journal of Medical Virology. 41. 170-173 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kimura H.et al.: "T-cell receptor Vα region usage in the cerebrospinal fluid of patients with mumps meningitis." Journal of Medical Virology. 41. 306-311 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 森島 恒雄: "単純ヘルペスウイルス" 脳と発達. 25. 124-127 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 森島 恒雄: "DNA増幅法による病原診断" 新小児医学大系ー小児医学の進歩ー. 92A. 229-236 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kimura H.他: "Relapse of herpes simplex encephalitis in children" Pediatrics. 89. 891-894 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 安藤 嘉浩 他: "PCR法による単純ヘルペス脳炎の早期診断" 小児感染免疫. 4. 19-22 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 早川 昌弘 他: "Polymerase chain reaction法にてサイトメガロウイルス 胎内感染を羊水診断した一例" 小児科学会雑誌. 96. 2349-2353 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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