| Project/Area Number |
04670716
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Psychiatric science
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| Research Institution | Tokyo Institute of Psychiatry |
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Principal Investigator |
IKEDA Kenji Tokyo Institute of Psychiatry, Research Scientist, 神経病理部門, 副参事研究員 (90232181)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | amyloidsubstance / toxic effect / neurofibrillary tangle / preamyloid / アルツハイマー病 / アミロイド / 老人斑 / 免疫組織化学 / タウタンパク / 微細構造 |
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| Research Abstract |
Toxic effect of amyloid substances against tissue was investigated. Diffuse type of senile plaques are thought to be an initial step of amyloid-formation. beta-amyloid or preamyloid in diffuse plaque was observed by electron microscopy applying immunohistochemical and methemanine-silver impregnation methods. The amyloid substance in this structure consisted of fine short fibrillar bundle and was partially amorphic. Most of the preamyloid deposited between small processes. The membrane of these processes showed unclear appearance in comparison to the same structure in other portion without preamyloid. This finding may indicate that the preamyloid induce the degeneration of the surrounding tissue due to its toxic effect.
Another important abnormal protein with amyloid nature, the neurofibrillary tangles (NFT) , was also investigated to elucidate its toxic effect. Extracellular NFT was, differ from intraneuronal NFT,surrounded by degenerated neuronal processes and invaded by numerous astrocytic processes and microglia. The Immunohistochemical and ultrastructural alteration of extracellular NFT was also noticed. A series of these findings may be the evidence of toxic effect of NFT against the surrounding tissue.
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