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AN IN VIVO MONITOR OF CHEMOTHERAPY FOR CANCER USING MULTI-NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Research Project

Project/Area Number 04670720
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionASAHIKAWA MEDICAL COLLEGE

Principal Investigator

MUNAKATA Tahashi  Asahikawa Med.College Assintant Professor, 医学部, 講師 (10166233)

Co-Investigator(Kenkyū-buntansha) SAKATA Hiromi  Asahikawa Med.College Research Associate, 医学部, 助手 (50235157)
KASAI Shinich  Asahikawa Med.College Associate Professor, 医学部, 助教授 (40091566)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywords19F NMR SPECTROSCOPY / 31P NMR SPECTROSCOPY / 5FU(Fluorouracil) / Fluoronucleotide / Fluoro-beta-alanin
Research Abstract

In the first year, we analyzed the basic parameters for the measurement of 19F NMR spectroscopy from the phantom in which 50mg/ml of 5FU was mounted. The apparatus was a superconducting NMR system named GX-270WB FT-NMR built by NIHON DENNSI Co.Ltd. 19F NMR spectra with Lorentzian curve were obtained under following parameters, resonant frequency ; 254.05MHz, line width ; 100kHz, Repetition time ; 1, . Furthermore we obtained spectra from perfused rat livers. The relationship between the strength of 19F signal and the intrahepatic concentration of fluorine was analyzed. Approximately 100nmol of fluorine was enough for the measurement within ten minutes. In the measurement of liver perfused with 5FU, changes in the level of 5FU and its metabolites i.e. FNucts, FUPA and FBAL were clearly observed. In the second year, with the basis of the results in 1st year, we tried to create a NMR probe and a coil for in vivo measurement. The first step was to examine the effects of the signal from skin and muscle between the surface coil and the liver. The distribution of signal intensity under the surface coil was measured using 1.Omm diameter micro sample tube in which the phosphoric acid solution was mounted. The region having a signal intensity 50% or more of maximal signal was 4.0 - 6.0 mm distance from the coil. This region well corresponds to the anatomical region of the liver. Since also exists near the coil at the edge of the coil, signals from muscle and skin were included the spectra. Surface coil method could detect the metabolic changes from the rat liver without surgical procedure. Under this condition, intrahepatic metabolic changes of the 5FU injected intravenously were clearly observed from anesthetized rat. It is concluded that combination of 31P and 19F NMR spectroscopy can simultaneously monitor the metabolism of anti-cancer drug and the changes in energy metabolism of cancer tissue.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Takashi Munakata: "The effects of Long-acting Somatostatin Analogue (octreotide) on Hepatic Energy Metabolism in Cirrhosis:A Clinical in-vivo 31P MRS Study.." Book of abstract:Society of Magnetic Resonance in Medicine. 3316-3316 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 棟方 隆: "肝硬変患者の^<31>P MRスペクトロスコピー-肝硬変重症度との関連について-" 日本外科学会雑誌 第93回臨時増刊号. 122-122 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takashi Munakata: "An in vivo ^<31>Phosphorus Magnetic Resonance Spectroscopy study of patients with liver cirrhosis:Progress towards a non-invasive assessment of disease severity." NMR in Biomedicine. Vol.6. 168-172 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takashi Munakata: "The effects of Octreotide on Hepatic Energy Metabolism in Cirrhosis." Book of abstract:2nd United European Gastroenterology Week. 758-758 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 棟方 隆: "MRSと肝・膵疾患 ^<31>P MR スペクトロスコピーの臨床応用" 肝・胆・膵. 28巻. 233-239 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Munakata, R.D.Griffiths, S.A.Jenkins, M.A.Martin, R.Shields and R.H.T.Edwards.: "The effects of long-acting somatostatin analogue (octreotide) on hepatic energy metabolism : A clinical in vivo 31P MRS Study." Book of abstruct : Society of Magnetic Resosnace in Medicine. 3316-3316 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Munakata, R.D.Griffiths, S.A.Jenkins, M.A.Martin, R.Shields and R.H.T.Edwards.: "Effects of long-acting somatostatin analogue on hepatic energy metabolism ; in vivo assessment of patients with liver cirrhosis using 31P MRS." Journal of Japan Surgical Society.Vol, 94. 122-122 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Munakata, R.D.Griffiths, P.A.Martin, S.A.Jenkins, R.Shields and R.H.T.Edwards.: "An in vivo P^<31> MRS Study of Patients With Liver Cirrhosis : Progress towards a Non-invasive Assessment of Disease Severity." NMR in Biomedicine.Vol.6. 168-172 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Munakata, R.D.Griffiths, P.A.Martin, R.Shields, R.H.T.Edwards and S.A.Jenkins.: "The Effects of Octreotide on Hepatic Energy Metabolism in Portal Hypertension." Book of abstruct : 2nd United European Gastroenterology Week. 758-758. 758-758 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Munakata: "Clinical in vivo 31P MR spectroscopy of the liver and pancreas" KAN.TAN.SUI.Vol.28. 233-239 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Takashi Munakata: "An in vivo ^<31>Phosphorus Magnetic Resonance Spectroscopy study of patients with liver cirrhosis:Progress towards a non-invasive assessment of disease severity." NMR in Biomedicine. Vol.6. 168-172 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 棟方 隆: "MRSと肝・膵疾患 ^<31>P MR スペクトロスコピーの臨床応用" 肝・胆・膵. 28巻. 233-239 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takashi Munakata: "The effects of Octreotide on Hepatic Energy Metabolism in Cirrhosis." Book of abstract:2nd United European Gastroenterology Week. 758-758 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takashi Munakata: "An in vivo ^<31>Phosphorus Magnetic Resonance Spectroscopy study of patients with liver cirrhosis:Progress towards a non-invasive assessment of disease severity." NMR in Biomedicine.

    • Related Report
      1992 Annual Research Report
  • [Publications] Takashi Munakata: "The effects of long-acting somatostatin anlogue(octreotide)on hepatic energy metabolism in cirrhosis:A clinical in-vivo ^<31>P MRS study." Book of abstract:Society of Magnetic Resonance in Medicine. vol2,. 3316-3316 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 棟方 隆: "肝硬変患者の^<31>P-MRスペクトロスコピーー肝硬変重症度との関連についてー" 日本外科学会雑誌. 第93回臨時増刊号. 122-122 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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