• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Detection of p53 gene mutations in fine-needle aspiration biopsy specimens from suspected breast cancers by polymerase chain reaction-single strand conformation polymorphism analysis.

Research Project

Project/Area Number 04670743
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Agro-economics
Research InstitutionSapporo Medical University School of Medicine

Principal Investigator

SATO Takashi  Sapporo Med.Univ.School of Medicine, Assistant prof., 医学部, 講師 (50205928)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Shuji  Sapporo Med.Univ.School of Medicine, Assistant prof., 医学部, 助手 (40231394)
OKAZAKI Akira  Sapporo Med.Univ.School of Medicine, Assistant prof., 医学部, 助手 (50213921)
OKAZAKI Minoru  Sapporo Med.Univ.School of Medicine, Assistant prof., 医学部, 講師 (30160667)
HIRATA Koichi  Sapporo Med.Univ.School of Medicine, Professor., 医学部, 教授 (50136959)
Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
KeywordsBreast cancer / Aspiration biopsy / PCR-SSCP / p53 / Point mutation / PCR / SSCP / P53
Research Abstract

The best means of reducing the mortality rate in the breast cancer patients is the early detection of breast cancers, and fine-needle aspirated breast tissue biopsy is one method to achieve this purpose. However, false-negative or false-positive diagnoses invariably occur and this can detract from the usefulness of the technique. Therefore, to obtain greater diagnostic accuracy with aspirated breast tissue specimens suspected of being cancers, we have evaluated the use of p53 gene mutation to identify cancer cells.
Genomic DNA was extracted from aspiration biopsy specimens taken from 15 suspected cases of breast cancer, including 7 known cases of breast cancer, and the p53 gene was studies for evidence of mutation by using a polymerase chain reaction-single strand conformation polymerphism (PCR-SSCP) analysis. In 5 of the 15 cases (33%), p53 gene mutation was identified and these tumors were subsequently histologically diagnosed as malignant. Further, DNA flow cytometry of the 15 tumors demonstrated that 6 (40%) were aneuploid and malignant, whereas 9 (60%) were diploid and benign. It was also found that the tumor cells in 5 aspirated cases that showed p53 gene mutations were all aneuploid, the p53 protein expression was positive, and the tumors were proved to be histologically malignant. It was thus concluded that the detection of p53 gene mutation by PCR-SSCP analysis of aspirated biopsy specimens from suspected breast cancers is a useful method for achieving a more accurate diagnosis.

Report

(4 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • 1992 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Takahashi,S.: "Correlation of heat shock protein 70 expression with estrogen receptor levels in invasive human breart cancer." Am.J.Clin.Pathol.101. 519-525 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato,T.: "Detection of p53 gene mutations and their protein overexpression in fine-needle biopsy specimens with talse-negative diagnoses in breast cancer." Tumor Res.29. 49-57 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato,T.: "Detection of p53 gene mutations in aspiration biopsy specimens from suspected breast cancers by polymerase chain reaction-single strand congormation..." Jpn.J.Cancer Res.,. 86. 140-145 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takahashi, S.: "Correlation of heat shock protein 70 expression with estrogen receptor levels in invasive human breast cancer." Am.J.Clin.Pathol.101. 519-525 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, T.: "Detection of p53 gene mutations and their protein overexpression in fine-needle biopsy specimens with false-negative diagnoses in breast cancer." Tumor Res.29. 49-57 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, T.: "Detection of p53 gene mutations in aspiration biopsy specimens from suspected breast cancers by polymerase chain reaction-single strand conformation polymorphism analysis." Jpn.J.Cancer Res.86. 140-145 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takahashi,S.,Sato,T.et al.: "Correlation of heat shock protein 70 expression with estrogen receptor levels in invasive human breast cancer." Am.J.Clin.Pathol.101. 519-525 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato,T.et al.: "Detection of p53 gene mutations and their protein overexpression in fine-needle biopsy specimens with false-negative diagnoses in breast cancer." Tumor Res.29(in press). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato,T.et al.: "Detection of p53 gene mutations in aspiration biopsy specimens from suspected breast cancers by polymerase chain reaction-single strand conformation…." Jpn.J.Cancer Res.86. 140-145 (1995)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi