| Project/Area Number |
04670766
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Dokkyo University School of Medicine (1993)
Tokyo Medical and Dental University (1992) |
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Principal Investigator |
SUNAGAWA Masakatsu Dokkyo Univ., Sch.of Med., Professor, 医学部, 教授 (10114761)
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| Co-Investigator(Kenkyū-buntansha) |
KAWANO Tatsuyuki Tokyo Med. & Dent.Univ., Assistant, 医学部, 助手 (00186115)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Esophageal SCC / C-erbB-2 / EGFR / P53 / MCC / DCC / DNA ploidy pattern / Prognosis of esophageal SCC / C-e〓bB-2 |
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| Research Abstract |
(1) Serum and tissue levels of c-erbB-2 oncoprotein of patient with esophageal squamas cell carcinoma : The c-erbB-2 concentration in sera and in cancerous tissues were examined by enzyme immunoassay (EIA). 11 of 29 (38%) cases were positive serum level oncoprotein which was over 13.5 U/ml protein. The mean consentration of c-erbB-2 protein in 14 malignant tissues was 61(〕SY.+-.〔)27.0 U/mg where as that of EGFR was 56.8(〕SY.+-.〔)27.6 fmol/mg. The concentration of c-erbB-2 protein was thought to correlate with that of EGFR.There was no relationship between concentration of c-erbB-2 protein and clinicopathological findings. The concentration of c-erbB-2 oncoprotein may be important indicator of prognosis of patient with esophageal S.C.C..
(2) Genetic alterations of p53, MCC and DCC in esophageal cancer tissues : Mutations in the DNA from 39 specimens of SCC were analyzed by PCR-SSCP method. The mutation of p53 was detected in 15 (38%) speciments, and that of MCC was in 12 (31%) and DCC wa
… More
s in 3 (8%). The primary tumor and metastasized lymph node were studied in the same 7 cases. Though the mutation of MCC was detected in 3 case of lymph nodes without mutation of main tumors, the mutations of p53 and DCC were found equally in the primary and secondary turmor. There was no relationship between the mutations and clinicopathological findings. The mutation of DCC was suggested to correlate with cancer development in late stage and in lymphogenous metastasis.
(3) A spectrophotometric study of DNA ploidy patterns of esophageal squamas cell carnicoma : DNA ploidy was determined by spectrophotometric analysis of paraffin embeded malignant tissue from 78 patient with S.C.C.. DNA distribution pattern was classified as diploid (D), low grade aneuploid (LGA) and high grade aneuploid (HGA) pattern. Out of 78 patients, 20 (26%) showed D, 15 (19%) LGA and 43 (55%) HGA.D was more frequent in early stage (70%). HGA was significantly higher frequency of lymph node metastases (79%), 5-year survival rate of D was 57%, where as HGA was significantly wors (14%). The role of the DNA ploidy pattern as a prognostic factor is emphasized. Less
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