| Project/Area Number |
04670774
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | GIFU UNIVERSITY |
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Principal Investigator |
IIDA Tatsumi GIFU UNIVERSITY HOSPITAL ASSISTANT PROFESSOR, 医学部・附属病院, 講師 (20222812)
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| Co-Investigator(Kenkyū-buntansha) |
SENGA Shohei GIFU UNIVERSITY HOSPITAL INSTRUCTOR, 医学部・附属病院, 助手 (20226690)
伊藤 英夫 岐阜大学, 医学部・附属病院, 医員
片桐 義文 岐阜大学, 医学部・附属病院, 医員
林 勝知 岐阜大学, 医学部, 講師 (80144020)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Fatty liver / Fepatic ischemia-reperfusion injury / L-SOD / SOD / Bile flow / High energy phosphate / Plasma amino acid profiles / 胆汁流量の回復率 / 虚血一再潅流 |
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| Research Abstract |
We have studied on the ischemic tolerance of the fatty liver in the rat. Recovery rate of bile flow after resperfusion (RRBF) is the good indicator of the viability of the warm-ischemically injured liver. Among the values of plasma free amino acids, alanine, glycine, methionine, aromatic amino acids and total plasma free amino acids correlate inversely to RRBF.These data suggested that these amino acids would be the good indicators of the viability of the warm-ischemically injured liver.
Liposomal encapsulated superoxide dismutase (L-SOD) contains superoxide dismutase, a highly specific scavenger of superoxide anion, in the liposomal encapsulation. By the administration of L-SOD, serum transaminases (GOT,GPT,LDH) are decreased and RRBF is increased after reperfusion. These data suggested that L-SOD has protective effect on the ischemically injured liver.
We have divided rats into 3 groupes to investigate the influence of the fatty deposition on the ischemic tolerance. Group 1 : normal liver group, group 2 : mild fatty liver group, group 3 : severe fatty liver group. All rats underwent hepatic ischemia for 60 minutes. One week survival after reperfusion in group 1 (100 %) and group 2 (75 %) are higher than in group 3 (0 %). RRBF in group 1 (52 %) is higher than in group 2 (34 %) and in group 2 is higher than in group 3 (11 %). Hepatic tissue ATP and total adenine nucleotides in group 1 are higher than in group 2 and group 3 at preischemia and 60 minutes after reperfusion.
These data suggested that :
Though mild fatty livers would not have ischemic resistances as much as normal livers, they have better ischemic resistance than severe fatty livers.
But energy state in fatty liver should be depressed compared with in normal liver even though the fatty deposit in liver is mild
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