A cancer-reactive human monoclonal antibody derived from a colonic cancer patient treated with localimmunotherapy
| Project/Area Number |
04670780
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
MONDEN Takushi Osaka University Assistant Professor, 医学部, 助手 (20174477)
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| Co-Investigator(Kenkyū-buntansha) |
OUE Masakuki Osaka University M.D., 医学部・付属病院, 医員
TAKEDA Tsutomu Osaka University Assistant Professor, 医学部, 助手 (80236471)
KOBAYASHI Tetsuro Osaka University Assistant Professor, 医学部, 助手 (40162002)
SHIMANO Takashi Osaka University Assistant Professor, 医学部, 講師 (80144476)
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| Project Period (FY) |
1992 – 1993
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| Project Status |
Completed (Fiscal Year 1993)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | human monoclonal antibody / local immunotherapy / colon cancer / cell surface antigen / sialylated lacto-series / 局所免疫療法 / in vivo stimmulation / ヒト型イムノグロブリン / がん特異性 |
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| Research Abstract |
A human monoclonal antibody, YJ-37 (IgM) was generated through thefusion of human B lymphoblastoid cell line HO-323 with the regional lymph node lymphocytes from a clonic cancer patient who was treated with a local immunotherapy. This antibody was purified and conjugated with biotin, after which direct immunohistochemical staining was performed. The results revealed that YJ-37 selectively reacted with colonic cancer (7/19), gastriccancer (7/13), but not with normal epithelia. Membrane immunofluorescence and FACS analysis also showed that YJ-37 bound to tumor cell surfaces. Furhermore, the chemical structure of the antigen defined by YJ-37 was analyzed by means of thin-layr chromatography immunostaining and ELSA.The results indicated that YJ-37 reacted with sialylated lacto-series carbohydrate chains, which have been reported to accumulate in cancer cells.
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Report
(3 results)
Research Products
(17 results)
