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Assessment of graft viability after cold preservation in small intestinal transplantation.

Research Project

Project/Area Number 04670785
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

TAGUCHI Tomoaki  M.D.Faculty of Medicine, Pediatric Surgery, Assistant Professor, 医学部, 助手 (20197247)

Co-Investigator(Kenkyū-buntansha) SUITA Sachiyo  M.D.Faculty of Medicine, Pediatric Surgery, Professor, 医学部, 教授 (30038856)
Project Period (FY) 1992 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordssmall intestine / transplantation / preservation / reperfusion / ATP / physiology / blood flow / histology / 再灌流 / 小腸移植 / 冷保存 / UW液 / エネルギー代謝 / レーザードップラー組織血流計 / 虚血再潅流傷害
Research Abstract

The precise assessment of graft viability is thought to be important, however there are still a few methods which remain controversial. We investigated the method for the assessment of small bowel graft viability, by means of physiological properties, energy metabolism, tissue blood flow, and the histological findings.
Syngeneic heterotopic transplantation was performed using Lewisrats, after a cold preservation of a jejunal graft with UW solution for 6, 12, 24, or 48hr. Graft samples were taken before transplantation for physiological study, and at harvesting, at the end of cold storage, before and 30 min after reperfusion for histology and for the measurement of adenine nucleotides (HPLC method). The tissue blood flow of the graft was recorded using a laserdoppler flowmeter at reperfusion.
The positive rates of samples showing both the biphasic nervous activities (excitation and inhibition) and the spontaneous rhythmic activity was 100%, 88%, and 25% in 0, 24, and 48 hr preservation, respectively. The level of ATP after reperfusion, which recovered to more than 30% of that at harvesting within 24 hr preservation groups but remained at less than 30% in the 48 hr group. The blood flow level at 5 or 30 min of reperfusion was significantly lower in the 48 hr group than that in the other groups. Histological damage after reperfusion, which extended deep into the crypt layr in the 48 hr group, but was confinded within villi in the other groups, correlated well with the results of physiology, energy metabolism, and tissu blood folw.
The physiological property before transplantation, and the tissue ATP level and blood flow after reperfusion are considered to be useful parameters for assessment of small bowel graft viability. These results are correlated with the recipient survival which we had previously reported.

Report

(3 results)
  • 1993 Annual Research Report   Final Research Report Summary
  • 1992 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Taguchi T: "Evaluation of UW solution for preservation of small intestinal transplants in the rat." Transplantation. 53. 1202-5 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Taguchi T: "Short segmental intestinal transplantation in rat-Functional comparison of jejunal and ileal grafts." Transplant Proc. 24. 1079-80 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirata Y: "Conversion of xanthine dehydrogenase to xanthine oxidase during ischemia of the rat small intestine and the effect of trifluoperazine on the conversion" J Pediatr Surg. 28. 597-600 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yamada T: "The assessment of small bowel graft viability based on energy metabolism and tissue blood flow" Transplant Proc.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Taguchi T: "A cytological examination of exudate on reperfusion as a parameter to evaluate graft viability in small bowel transplantation" Transplant Proc.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Taguchi T: "Parameter for graft viability before transplantation in the rat small bowel -A physiological evaluation of a preserved graft-" Transplant Proc.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Taguchi T et al.: "Evaluation of UW solution for preservation of small intestinal transplants in the rat." Transplantation. 53. 1202-5 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Taguchi T et al.: "Short segmental intestinal transplantation in rat-Functional comparison of jejunal and ileal grafts." Transplant Proc. 24. 1079-80 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hirata Y et al.: "Conversion of xanthine dehydrogenase to xanthine oxidase during ischemia of the rat small intestine and the effect of trifluoperazine on the conversion" J Pediatr Surg. 28. 597-600 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yamada T et al.: "The assessment of small bowel graft viability based on energy metabolism and tissue blood flow" Transplant Proc. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Taguchi T et al.: "A cytological examination of exudate on reperfusion, as a parameter to evaluate graft viability in small bowel transplantation." Transplant Proc. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Taguchi T et al.: "Parameter for graft viability before transplantation in the rat small bowel -A physiological evaluation of a preserved graft-." Transplant Proc. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Taguchi T: "Evaluation of UW solution for preservation of small intestinal trasplants in the rat." Transplantation. 53. 1202-5 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Taguchi T: "Short segmental intestinal transplantation in rat-Functional comparison of jejunal and ileal grafts." Transplant Proc. 24. 1079-80 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hirata Y: "Conversion of xanthine dehydrogenase to xanthine oxidase during ischemia of the rat small intestine and the effect of trifluoperazine on the conversion." J Pediatr Surg. 28. 597-600 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamada T: "The assessment of small bowel graft viability based on energy metabolism and tissue blood flow." Transplant Proc,. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Taguchi T: "A cytological examination of exudate on reperfusion,as a parameter to evaluate graft viability in small bowel transplantation." Transplant Proc,. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] Taguchi T: "Parameter for graft viability before transplantation in the rat small bowel -A physiological evaluation of a preserved graft-" Transplant Proc,. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Taguchi,Y.Hirata,R.Hirose,T.Yamada,S.Suita: "Short Segmental Intestinal Transplantation in Rats-Functional Comparison of Jejunal and Ileal grafts" Transplantation Proceedings. 24. 1079-80 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] R.Hirose,T.Taguchi,Y.Hirata,T.Yamada,S.Suita,O.Nada: "Patterns of Enteric Nervous Distribution After Small Bowel Transplantation" Transplantation Proceedings. 24. 1108-9 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Taguchi,E.Zorychta,FM.Guttman: "Evaluation of UWsolution for Preservation of Small Intestinal Transplants in the Rat" Transplantation. 53. 1202-5 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 田口 智章,山田 耕治,平田 祐造,廣瀬 龍一郎,水田 祥代,豊原 敏光: "マイクロサージェリーを用いたラット小腸移植におけるグラフト採取法に関する検討" 日本小児外科学会雑誌. 28. 1314-20 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 田口 智章,水田 祥代: "小腸移植における移植小腸の採取部位と長さ" 医学のあゆみ. 163. 826-8 (1992)

    • Related Report
      1992 Annual Research Report

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Published: 1992-04-01   Modified: 2016-04-21  

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