| Project/Area Number |
04670787
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
KUWANO Hiroyuki FACULTY OF MEDICINE,KYUSHU UNIVERSITY.ASSISTANT PROFESSOR, 医学部, 講師 (90186560)
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| Co-Investigator(Kenkyū-buntansha) |
定永 倫明 九州大学, 医学部, 医員
池部 正彦 九州大学, 医学部, 医員
安達 洋祐 九州大学, 医学部, 助手 (90221043)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Esophageal Cancer / Carcinogenesis / Genetic change / dysplasia / p53 / In situ hybridization / Type IV collagenase / TypeIV collagenase / 浸潤 / TypeIVcollagenase / In situ hybridization / 癌遺伝子 / c-myc / 免疫組織化学 / 分子生物学 |
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| Research Abstract |
The purpose of this study is to clarify biological features of carcinogenesis and progression of esophageal carcinoma with investigating the distribution of carcinoma-related gene expression by immunohistochemistry or in situ hybridization.
Regarding carcinogenesis, expression of tumor suppressor p53 protein in esophageal squamous dysplasia, which is considered to be a putative precancerous lesion of esophageal carcinoma, was immunohistochemically investigated. As regards progression, expression of Type IV collagenase, which is one of metalloproteinases and is reported to participate in progression or invasion of many kinds of carcinomas, was investigated in cases with esopahgeal carcinoma by in situ hybridization.
As a result of the former study, mutation of p53 gene was detected in atypical cell layr of esophageal squamous dysplasia similar to that of carcinoma in cases with continuity between dysplasia and carcinoma. This result suggested that squamous dysplasia of the esophagus is a 'subcancerous lesion' rather than a 'precancerous lesion'.
Expression of Type IV collagenase was detected on the membrane of a few cells in each nest of carcinoma, and the degree of intensity was dependent upon stage of the carcinoma. This result suggested that expression of this enzyme participated in progression of esophageal carcinoma.
In the near future, it will be expected that investigation of other genetic chages with the same technique will make details of biological features of carcinogenesis and progression of esophageal carcinoma more clear.
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